Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae

Kazuo Yamagishi, Minetaka Sugiyama, Yoshinobu Kaneko, Masafumi Nishizawa, Satoshi Harashima

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

Original languageEnglish
Pages (from-to)563-567
Number of pages5
JournalJournal of Bioscience and Bioengineering
Issue number6
Publication statusPublished - 2008 Dec 1


  • Cre-loxP system
  • budding yeast
  • chromosome instability
  • chromosome loss
  • chromosome splitting
  • mini-chromosome

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this