Construction of a metagenomic library for the marine sponge Halichondria okadai

Takahiro Abe, Fatma Pinar Sahin, Kiyotaka Akiyama, Takayuki Naito, Mizoe Kishigami, Kenji Miyamoto, Yasufumi Sakakibara, Daisuke Uemura

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454- pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alphaproteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria, and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.

Original languageEnglish
Pages (from-to)633-639
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Issue number4
Publication statusPublished - 2012


  • 454-pyrosequencing
  • Fosmid
  • Halichondria okadai
  • Metagenomic library
  • Sponge

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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