Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase

Masafumi Nishizawa; Fumiko Ozawa; Fumio Hishinuma

doi:10.1007/BF00184982

Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase

Masafumi Nishizawa, Fumiko Ozawa, Fumio Hishinuma

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage site or a Lys-Arg sequence which is a cleavage site for the product of the KEX2 gene. Both vectors were able to direct the expression and secretion of mouse amylase. One of them has a SalI site within the signal sequence, and an attempt to clone sequences enhancing secretion of amylase with this vector is reported.

Original language	English
Pages (from-to)	317-322
Number of pages	6
Journal	Applied Microbiology and Biotechnology
Volume	32
Issue number	3
DOIs	https://doi.org/10.1007/BF00184982
Publication status	Published - 1989 Dec 1

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Access to Document

10.1007/BF00184982

Cite this

@article{3b0970d2a4f74b0d882d93e3deb9b1fd,

title = "Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase",

abstract = "Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage site or a Lys-Arg sequence which is a cleavage site for the product of the KEX2 gene. Both vectors were able to direct the expression and secretion of mouse amylase. One of them has a SalI site within the signal sequence, and an attempt to clone sequences enhancing secretion of amylase with this vector is reported.",

author = "Masafumi Nishizawa and Fumiko Ozawa and Fumio Hishinuma",

year = "1989",

month = dec,

day = "1",

doi = "10.1007/BF00184982",

language = "English",

volume = "32",

pages = "317--322",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer Verlag",

number = "3",

}

TY - JOUR

T1 - Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase

AU - Nishizawa, Masafumi

AU - Ozawa, Fumiko

AU - Hishinuma, Fumio

PY - 1989/12/1

Y1 - 1989/12/1

N2 - Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage site or a Lys-Arg sequence which is a cleavage site for the product of the KEX2 gene. Both vectors were able to direct the expression and secretion of mouse amylase. One of them has a SalI site within the signal sequence, and an attempt to clone sequences enhancing secretion of amylase with this vector is reported.

AB - Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage site or a Lys-Arg sequence which is a cleavage site for the product of the KEX2 gene. Both vectors were able to direct the expression and secretion of mouse amylase. One of them has a SalI site within the signal sequence, and an attempt to clone sequences enhancing secretion of amylase with this vector is reported.

UR - http://www.scopus.com/inward/record.url?scp=0024836520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024836520&partnerID=8YFLogxK

U2 - 10.1007/BF00184982

DO - 10.1007/BF00184982

M3 - Article

AN - SCOPUS:0024836520

SN - 0175-7598

VL - 32

SP - 317

EP - 322

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 3

ER -

Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this