TY - GEN
T1 - Control of burn wound sepsis in rats by methylene blue-mediated photodynamic treatment
AU - Hasegawa, Hiroyuki
AU - Sato, Shunichi
AU - Kawauchi, Satoko
AU - Saitoh, Daizoh
AU - Shinomiya, Nariyoshi
AU - Ashida, Hiroshi
AU - Terakawa, Mitsuhiro
PY - 2012
Y1 - 2012
N2 - Control of wound sepsis is an important challenge in traumatology. However, increase in the drug-resistant bacteria makes this challenge considerably difficult in recent years. In this study, we attempted to control burn wound sepsis in rats by photodynamic treatment, which has been reported to be effective against some drug-resistant bacteria. A 20% TBSA (total body surface area) full-thickness burn was made in rat dorsal skin, and five days after injury, a suspension of P. aeruginosa was applied to the wound surface. At 30 min after infection, a methylene blue (MB) solution was applied to the wound surface; 5 min afterwards, the wound was illuminated with a 665-nm light emitting diode (LED) array for 10 min. This treatment (application of MB and illumination) was repeated 3 times successively. The averaged light intensity on the wound surface was 3.3 mW/cm 2, the corresponding total light dose being 5.9 J/cm 2. One week after injury, the numbers of bacteria in the blood and liver were counted by colony forming assay. In the liver, the number of bacteria of the treated group was significantly lower than that of the sham control group without photodynamic treatment. In the blood, no bacteria were detected in the treated group, while a certain amount of bacteria was detected in the control group. These results demonstrate the efficacy of MB-mediated PDT with a red LED array to control burn wound sepsis.
AB - Control of wound sepsis is an important challenge in traumatology. However, increase in the drug-resistant bacteria makes this challenge considerably difficult in recent years. In this study, we attempted to control burn wound sepsis in rats by photodynamic treatment, which has been reported to be effective against some drug-resistant bacteria. A 20% TBSA (total body surface area) full-thickness burn was made in rat dorsal skin, and five days after injury, a suspension of P. aeruginosa was applied to the wound surface. At 30 min after infection, a methylene blue (MB) solution was applied to the wound surface; 5 min afterwards, the wound was illuminated with a 665-nm light emitting diode (LED) array for 10 min. This treatment (application of MB and illumination) was repeated 3 times successively. The averaged light intensity on the wound surface was 3.3 mW/cm 2, the corresponding total light dose being 5.9 J/cm 2. One week after injury, the numbers of bacteria in the blood and liver were counted by colony forming assay. In the liver, the number of bacteria of the treated group was significantly lower than that of the sham control group without photodynamic treatment. In the blood, no bacteria were detected in the treated group, while a certain amount of bacteria was detected in the control group. These results demonstrate the efficacy of MB-mediated PDT with a red LED array to control burn wound sepsis.
KW - Burn wound
KW - Infection
KW - LED
KW - Methylene blue
KW - Photodynamic therapy
KW - Pseudomonas aeruginosa
KW - Sepsis
KW - Septicemia
UR - http://www.scopus.com/inward/record.url?scp=84859320078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859320078&partnerID=8YFLogxK
U2 - 10.1117/12.907643
DO - 10.1117/12.907643
M3 - Conference contribution
AN - SCOPUS:84859320078
SN - 9780819488534
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Methods for Tumor Treatment and Detection
T2 - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI
Y2 - 21 January 2012 through 22 January 2012
ER -