TY - GEN
T1 - Control of cellular organization around collagen beads using dielectrophoresis
AU - Miyata, Shogo
AU - Sugimoto, Yu
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Tissues formed by cells seeded in hydrogels are used for biotechnology, cell-based assays, and tissue engineering. We have previously presented a cell-micropatterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces, and have demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen micro-beads. As a case study, we produce collagen-alginate beads for scaffold and accumulate chondrocytes around the micro-beads by combining DEP force and flow of the buffer solution. This approach enables an expanded range of studies examining the influence of 3D cellular architecture on micro-scale levels.
AB - Tissues formed by cells seeded in hydrogels are used for biotechnology, cell-based assays, and tissue engineering. We have previously presented a cell-micropatterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces, and have demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen micro-beads. As a case study, we produce collagen-alginate beads for scaffold and accumulate chondrocytes around the micro-beads by combining DEP force and flow of the buffer solution. This approach enables an expanded range of studies examining the influence of 3D cellular architecture on micro-scale levels.
KW - Alginate
KW - Cellular organization
KW - Collagen
KW - Dielectrophoresis
UR - http://www.scopus.com/inward/record.url?scp=78651459718&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651459718&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:78651459718
SN - 9781424496730
T3 - 2010 World Automation Congress, WAC 2010
BT - 2010 World Automation Congress, WAC 2010
T2 - 2010 World Automation Congress, WAC 2010
Y2 - 19 September 2010 through 23 September 2010
ER -