@article{216cb8e7484c4e21bf16bbd71390eda9,
title = "Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator",
abstract = "We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution.",
author = "Hirotaka Kanuka and Kazunobu Sawamoto and Naohiro Inohara and Kenji Matsuno and Hideyuki Okano and Masayuki Miura",
note = "Funding Information: We are grateful to Tatsushi Igaki and Ritsuko Shimamura for technical support, Xiaodong Wang for human Apaf-1 cDNA, Shigeomi Shimizu for cytochrome c, Kumiko Ui-Tei for G3PDH primer sets, Hermann Steller and Bruce Hay for fly stocks, the Berkeley Drosophila Genome Project for providing various reagents and informations, and the Bloomington Stock Center for fly stocks. This work was supported in part by grants from the Japanese Ministry of Education, Science, Sports and Culture to H. O. and M. M., and from SPSBS, Science and Technology Agency of Japan to K. S. This work was also supported in part to H. O. by Core Research for Evolutionary Science and Technology (CREST), Japan Science and Technology Corporation. H. K. is a research fellow of the Japan Society for the Promotion of Science. ",
year = "1999",
month = nov,
doi = "10.1016/S1097-2765(00)80386-X",
language = "English",
volume = "4",
pages = "757--769",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "5",
}