Rei Murata, Yuki Takada, Hiroyuki Takuwa, Hiroshi Kawaguchi, Hiroshi Ito, Iwao Kanno, Naotomo Tottori, Yukio Yamada, Yutaka Tomita, Yoshiaki Itoh, Norihiro Suzuki, Katsuya Yamada, Kazuto Masamoto

Research output: Contribution to journalArticlepeer-review


The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalAdvances in experimental medicine and biology
Publication statusPublished - 2014
Externally publishedYes


  • 2- NBDG
  • Glucose transporter
  • Optical imaging
  • Two-photon microscopy
  • Vascular endothelial cell

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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