TY - JOUR
T1 - Creating a Saccharomyces cerevisiae haploid strain having 21 chromosomes
AU - Widianto, Donny
AU - Yamamoto, Eishi
AU - Sugiyama, Minetaka
AU - Mukai, Yukio
AU - Kaneko, Yoshinobu
AU - Oshima, Yasuji
AU - Nishizawa, Masafumi
AU - Harashima, Satoshi
N1 - Funding Information:
This study was partially supported by the Ministry of Education, Culture, Sports, Science and Technology, a Grant-in-Aid for Scientific Research B, 12460044, 2000 to 2002, and carried out as a part of The Project for Development of a Technological Infrastructure for Industrial Bioprocesses on R & D of New Industrial Science and Technology Frontiers by the Ministry of Economy, Trade and Industry (METI), and entrusted by the New Energy and Industrial Technology Development Organization (NEDO).
PY - 2003
Y1 - 2003
N2 - Chromosome engineering techniques that can manipulate a large segment of chromosomal DNA are useful not only for studying the organization of eukaryotic genomes but also for the improvement of industrially important strains. Toward the development of techniques that can efficiently manipulate a large segment of chromosome, we have previously reported a one-step chromosome splitting technique in a haploid Saccharomyces cerevisiae cell, with which we could successfully split yeast chromosome II, XIII, or XI into two halves to create a haploid strain having 17 chromosomes. We have now constructed chromosome splitting vectors bearing ADE2, HIS3, LEU2, or TRP1 marker, and by using these vectors, we could successively split yeast chromosomes to create a novel yeast haploid strain having up to 21 chromosomes. The specific growth rates of yeast strains carrying more than 16 chromosomes up to 21 did not differ significantly, suggesting that yeast cells can harbor more chromosomes than they do in their natural state, that is, 16 chromosomes, without serious effects on their growth.
AB - Chromosome engineering techniques that can manipulate a large segment of chromosomal DNA are useful not only for studying the organization of eukaryotic genomes but also for the improvement of industrially important strains. Toward the development of techniques that can efficiently manipulate a large segment of chromosome, we have previously reported a one-step chromosome splitting technique in a haploid Saccharomyces cerevisiae cell, with which we could successfully split yeast chromosome II, XIII, or XI into two halves to create a haploid strain having 17 chromosomes. We have now constructed chromosome splitting vectors bearing ADE2, HIS3, LEU2, or TRP1 marker, and by using these vectors, we could successively split yeast chromosomes to create a novel yeast haploid strain having up to 21 chromosomes. The specific growth rates of yeast strains carrying more than 16 chromosomes up to 21 did not differ significantly, suggesting that yeast cells can harbor more chromosomes than they do in their natural state, that is, 16 chromosomes, without serious effects on their growth.
KW - Chromosome splitting
KW - Genome technology
KW - Yeast
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U2 - 10.1263/jbb.95.89
DO - 10.1263/jbb.95.89
M3 - Article
C2 - 16233372
AN - SCOPUS:0037278680
SN - 1389-1723
VL - 95
SP - 89
EP - 94
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -