Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis

Nobuyuki Takakura; Xu Ling Huang; Takeshi Naruse; Isao Hamaguchi; Daniel J. Dumont; George D. Yancopoulos; Toshio Suda

doi:10.1016/S1074-7613(00)80665-2

Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis

Nobuyuki Takakura, Xu Ling Huang, Takeshi Naruse, Isao Hamaguchi, Daniel J. Dumont, George D. Yancopoulos, Toshio Suda

Research output: Contribution to journal › Article › peer-review

264 Citations (Scopus)

Abstract

We have investigated the function of TIE2/TEK receptor tyrosine kinase in the development of definitive hematopoiesis. In the vitelline artery at 9.5 days post-coitum (d.p.c.), TIE2⁺ hematopoietic cells aggregated and adhered to TIE2⁺ endothelial cells. Soluble TIE2-Fc chimeric protein inhibited the development of hematopoiesis and angiogenesis in the para- aortic splanchnopleural mesoderm (P-Sp) explant culture, and TIE2-deficient mice showed severely impaired definitive hematopoiesis. An in vitro study revealed that Angiopoietin-1 but not Angiopoietin-2 promoted the adhesion to fibronectin (FN) through integrins in TIE2-transfected cells and primary TIE2⁺ cells sorted from 9.5 d.p.c. P-Sp. Adhesion of TIE2⁺ cells induced by Angiopoietin-1 enhanced the proliferation of hematopoietic progenitor cells.

Original language	English
Pages (from-to)	677-686
Number of pages	10
Journal	Immunity
Volume	9
Issue number	5
DOIs	https://doi.org/10.1016/S1074-7613(00)80665-2
Publication status	Published - 1998 Nov
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1074-7613(00)80665-2

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@article{08f37968204e4d4b963ccda01528006a,

title = "Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis",

abstract = "We have investigated the function of TIE2/TEK receptor tyrosine kinase in the development of definitive hematopoiesis. In the vitelline artery at 9.5 days post-coitum (d.p.c.), TIE2+ hematopoietic cells aggregated and adhered to TIE2+ endothelial cells. Soluble TIE2-Fc chimeric protein inhibited the development of hematopoiesis and angiogenesis in the para- aortic splanchnopleural mesoderm (P-Sp) explant culture, and TIE2-deficient mice showed severely impaired definitive hematopoiesis. An in vitro study revealed that Angiopoietin-1 but not Angiopoietin-2 promoted the adhesion to fibronectin (FN) through integrins in TIE2-transfected cells and primary TIE2+ cells sorted from 9.5 d.p.c. P-Sp. Adhesion of TIE2+ cells induced by Angiopoietin-1 enhanced the proliferation of hematopoietic progenitor cells.",

author = "Nobuyuki Takakura and Huang, {Xu Ling} and Takeshi Naruse and Isao Hamaguchi and Dumont, {Daniel J.} and Yancopoulos, {George D.} and Toshio Suda",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, and Culture of Japan. The authors thank Hiroaki Kodama for providing us with OP9 stromal cells.",

year = "1998",

month = nov,

doi = "10.1016/S1074-7613(00)80665-2",

language = "English",

volume = "9",

pages = "677--686",

journal = "Immunity",

issn = "1074-7613",

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T1 - Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis

AU - Takakura, Nobuyuki

AU - Huang, Xu Ling

AU - Naruse, Takeshi

AU - Hamaguchi, Isao

AU - Dumont, Daniel J.

AU - Yancopoulos, George D.

AU - Suda, Toshio

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, and Culture of Japan. The authors thank Hiroaki Kodama for providing us with OP9 stromal cells.

PY - 1998/11

Y1 - 1998/11

N2 - We have investigated the function of TIE2/TEK receptor tyrosine kinase in the development of definitive hematopoiesis. In the vitelline artery at 9.5 days post-coitum (d.p.c.), TIE2+ hematopoietic cells aggregated and adhered to TIE2+ endothelial cells. Soluble TIE2-Fc chimeric protein inhibited the development of hematopoiesis and angiogenesis in the para- aortic splanchnopleural mesoderm (P-Sp) explant culture, and TIE2-deficient mice showed severely impaired definitive hematopoiesis. An in vitro study revealed that Angiopoietin-1 but not Angiopoietin-2 promoted the adhesion to fibronectin (FN) through integrins in TIE2-transfected cells and primary TIE2+ cells sorted from 9.5 d.p.c. P-Sp. Adhesion of TIE2+ cells induced by Angiopoietin-1 enhanced the proliferation of hematopoietic progenitor cells.

AB - We have investigated the function of TIE2/TEK receptor tyrosine kinase in the development of definitive hematopoiesis. In the vitelline artery at 9.5 days post-coitum (d.p.c.), TIE2+ hematopoietic cells aggregated and adhered to TIE2+ endothelial cells. Soluble TIE2-Fc chimeric protein inhibited the development of hematopoiesis and angiogenesis in the para- aortic splanchnopleural mesoderm (P-Sp) explant culture, and TIE2-deficient mice showed severely impaired definitive hematopoiesis. An in vitro study revealed that Angiopoietin-1 but not Angiopoietin-2 promoted the adhesion to fibronectin (FN) through integrins in TIE2-transfected cells and primary TIE2+ cells sorted from 9.5 d.p.c. P-Sp. Adhesion of TIE2+ cells induced by Angiopoietin-1 enhanced the proliferation of hematopoietic progenitor cells.

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Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis

Abstract

ASJC Scopus subject areas

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