TY - JOUR
T1 - Cryogenic coherent x-ray diffraction imaging for biological non-crystalline particles using the KOTOBUKI-1 diffraction apparatus at SACLA
AU - Oroguchi, Tomotaka
AU - Sekiguchi, Yuki
AU - Kobayashi, Amane
AU - Masaki, Yu
AU - Fukuda, Asahi
AU - Hashimoto, Saki
AU - Nakasako, Masayoshi
AU - Ichikawa, Yuichi
AU - Kurumizaka, Hitoshi
AU - Shimizu, Mitsuhiro
AU - Inui, Yayoi
AU - Matsunaga, Sachihiro
AU - Kato, Takayuki
AU - Namba, Keiichi
AU - Yamaguchi, Keiichi
AU - Kuwata, Kazuo
AU - Kameda, Hiroshi
AU - Fukui, Naoya
AU - Kawata, Yasushi
AU - Kameshima, Takashi
AU - Takayama, Yuki
AU - Yonekura, Koji
AU - Yamamoto, Masaki
N1 - Publisher Copyright:
� 2015 IOP Publishing Ltd.
PY - 2015/7/29
Y1 - 2015/7/29
N2 - We have developed an experimental apparatus named KOTOBUKI-1 for use in the coherent x-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. The apparatus allows us to collect diffraction data for frozen-hydrated specimens at 66 K and provides an experimental environment to easily transfer frozen-hydrated specimens from liquid nitrogen storage to the specimen stage for x-ray exposure. Since 2012, the apparatus has been used in the single-shot diffraction data collection of non-crystalline biological cells and cellular components with dimensions from micrometer to submicrometer using x-ray free electron lasers at SACLA. Here we report on the performance of the KOTOBUKI-1 diffraction apparatus and some structure analyses of biological cells and cellular components. Based on the present results, we also discuss the future developments of diffraction apparatus for more efficient data collection.
AB - We have developed an experimental apparatus named KOTOBUKI-1 for use in the coherent x-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. The apparatus allows us to collect diffraction data for frozen-hydrated specimens at 66 K and provides an experimental environment to easily transfer frozen-hydrated specimens from liquid nitrogen storage to the specimen stage for x-ray exposure. Since 2012, the apparatus has been used in the single-shot diffraction data collection of non-crystalline biological cells and cellular components with dimensions from micrometer to submicrometer using x-ray free electron lasers at SACLA. Here we report on the performance of the KOTOBUKI-1 diffraction apparatus and some structure analyses of biological cells and cellular components. Based on the present results, we also discuss the future developments of diffraction apparatus for more efficient data collection.
KW - Biological cell
KW - Coherent x-ray diffraction imaging
KW - X-ray free electron laser
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U2 - 10.1088/0953-4075/48/18/184003
DO - 10.1088/0953-4075/48/18/184003
M3 - Article
AN - SCOPUS:84945152204
SN - 0953-4075
VL - 48
JO - Journal of Physics B: Atomic, Molecular and Optical Physics
JF - Journal of Physics B: Atomic, Molecular and Optical Physics
IS - 18
ER -