Cyclic AMP-dependent PKA phosphorylates starfish sperm proteins during acrosome reaction

M. Sadiqul Islam; Motonori Hoshi; Midori Matsumoto

doi:10.2478/s11535-007-0001-6

Cyclic AMP-dependent PKA phosphorylates starfish sperm proteins during acrosome reaction

M. Sadiqul Islam, Motonori Hoshi, Midori Matsumoto

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The induction of acrosome reaction (AR) happens when starfish spermatozoa encounter the egg jelly (EJ). This complex process involves different signal transduction pathways, such as elevation of cAMP and the activation of protein kinase A (PKA). The specific inhibitors of PKA (H89 and KT5720) have been shown to inhibit the EJ-induced Ca²⁺ elevation and AR. By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ. The specific PKA inhibitors effectively inhibit phosphorylation of these proteins, suggesting an involvement of PKA on EJ-induced AR.

Original language	English
Pages (from-to)	109-121
Number of pages	13
Journal	Central European Journal of Biology
Volume	2
Issue number	1
DOIs	https://doi.org/10.2478/s11535-007-0001-6
Publication status	Published - 2007 Mar

Keywords

Acrosome reaction
PKA
Phosphorylation
Spermatozoa
Starfish

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)

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10.2478/s11535-007-0001-6

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title = "Cyclic AMP-dependent PKA phosphorylates starfish sperm proteins during acrosome reaction",

abstract = "The induction of acrosome reaction (AR) happens when starfish spermatozoa encounter the egg jelly (EJ). This complex process involves different signal transduction pathways, such as elevation of cAMP and the activation of protein kinase A (PKA). The specific inhibitors of PKA (H89 and KT5720) have been shown to inhibit the EJ-induced Ca2+ elevation and AR. By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ. The specific PKA inhibitors effectively inhibit phosphorylation of these proteins, suggesting an involvement of PKA on EJ-induced AR.",

keywords = "Acrosome reaction, PKA, Phosphorylation, Spermatozoa, Starfish",

author = "Islam, {M. Sadiqul} and Motonori Hoshi and Midori Matsumoto",

note = "Funding Information: We thank the Director and staff members of the Otsuchi Marine Research Center and the Misaki Marine Biological Station, the University of Tokyo for their help in collecting the starfish. This research was partially supported by the Grant-in-Aid for the 21st Century Center of Excellence (COE) Program entitled “Understanding and Control of Life{\textquoteright}s Function via System Biology (Keio University)” (to M.S.I.).",

year = "2007",

month = mar,

doi = "10.2478/s11535-007-0001-6",

language = "English",

volume = "2",

pages = "109--121",

journal = "Central European Journal of Biology",

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AU - Islam, M. Sadiqul

AU - Hoshi, Motonori

AU - Matsumoto, Midori

N1 - Funding Information: We thank the Director and staff members of the Otsuchi Marine Research Center and the Misaki Marine Biological Station, the University of Tokyo for their help in collecting the starfish. This research was partially supported by the Grant-in-Aid for the 21st Century Center of Excellence (COE) Program entitled “Understanding and Control of Life’s Function via System Biology (Keio University)” (to M.S.I.).

PY - 2007/3

Y1 - 2007/3

N2 - The induction of acrosome reaction (AR) happens when starfish spermatozoa encounter the egg jelly (EJ). This complex process involves different signal transduction pathways, such as elevation of cAMP and the activation of protein kinase A (PKA). The specific inhibitors of PKA (H89 and KT5720) have been shown to inhibit the EJ-induced Ca2+ elevation and AR. By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ. The specific PKA inhibitors effectively inhibit phosphorylation of these proteins, suggesting an involvement of PKA on EJ-induced AR.

AB - The induction of acrosome reaction (AR) happens when starfish spermatozoa encounter the egg jelly (EJ). This complex process involves different signal transduction pathways, such as elevation of cAMP and the activation of protein kinase A (PKA). The specific inhibitors of PKA (H89 and KT5720) have been shown to inhibit the EJ-induced Ca2+ elevation and AR. By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ. The specific PKA inhibitors effectively inhibit phosphorylation of these proteins, suggesting an involvement of PKA on EJ-induced AR.

KW - Acrosome reaction

KW - PKA

KW - Phosphorylation

KW - Spermatozoa

KW - Starfish

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SN - 1895-104X

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JO - Central European Journal of Biology

JF - Central European Journal of Biology

IS - 1

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Cyclic AMP-dependent PKA phosphorylates starfish sperm proteins during acrosome reaction

Abstract

Keywords

ASJC Scopus subject areas

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