TY - JOUR
T1 - Cytokine Levels in the Aqueous Humor Are Associated With Corneal Thickness in Eyes With Bullous Keratopathy
AU - Suzuki, Natsume
AU - Yamaguchi, Takefumi
AU - Shibata, Shinsuke
AU - Nagai, Toshihiro
AU - Noma, Hisashi
AU - Tsubota, Kazuo
AU - Shimazaki, Jun
N1 - Funding Information:
Funding/Support: This study was supported by a Grant-in-Aid for Scientific Research (15K10906) from the Ministry of Education, Culture, Sports, Science and Technology and by a Grant for Brain/MINDS by AMED (JP17dm0207002) (Shinsuke Shibata). The following authors have no financial disclosures: Natsume Suzuki, Toshihiro Nagai, Hisashi Noma, Kazuo Tsubota, Jun Shimazaki. All authors attest that they meet the current ICMJE criteria for authorship.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/2
Y1 - 2019/2
N2 - Purpose: We sought to investigate the association between the severity of bullous keratopathy and proinflammatory cytokine levels in the aqueous humor (AqH). Design: Cross-sectional study. Methods: This study included a total of 95 eyes: 62 with bullous keratopathy and 33 that underwent cataract surgery. Central corneal thickness (CCT) and central corneal volume within 4 and 6 mm (CCV4mm and CCV6mm, respectively) were determined using anterior segment optical coherence tomography. A total of 95 AqH samples were collected at the beginning of surgery. The levels of cytokines (interleukins [ILs]-1α -1β -4, -6, -8, -10, -12p70, -13, -17A, interferon [IFN]-α IFN-γ monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule-1 [sICAM-1]) in the AqH were measured using multiplex beads immunoassay. We evaluated the correlation among AqH cytokine levels, CCT, CCV4mm, and CCV6mm in eyes with bullous keratopathy. Results: The levels of protein, ILs-4, -6, -8, -10, -12p70, and -17A, MCP-1, IFN-γ E-selectin, P-selectin, and sICAM-1 were significantly higher in eyes with bullous keratopathy compared with those of the normal control subjects (all P <.0025). CCT was significantly correlated with the levels of IL-13 (r = 0.551, P =.0009) and sICAM-1 (r = 0.448, P =.0005). CCV4mm was significantly correlated with the levels of IL-13 (r = 0.514, P =.0022) and sICAM-1 (r = 0.404, P =.0019). CCV6mm was significantly correlated with the level of sICAM-1 (r = 0.459, P =.0003). Conclusion: The severity of corneal edema in eyes with bullous keratopathy was associated with the levels of specific cytokines in the AqH.
AB - Purpose: We sought to investigate the association between the severity of bullous keratopathy and proinflammatory cytokine levels in the aqueous humor (AqH). Design: Cross-sectional study. Methods: This study included a total of 95 eyes: 62 with bullous keratopathy and 33 that underwent cataract surgery. Central corneal thickness (CCT) and central corneal volume within 4 and 6 mm (CCV4mm and CCV6mm, respectively) were determined using anterior segment optical coherence tomography. A total of 95 AqH samples were collected at the beginning of surgery. The levels of cytokines (interleukins [ILs]-1α -1β -4, -6, -8, -10, -12p70, -13, -17A, interferon [IFN]-α IFN-γ monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule-1 [sICAM-1]) in the AqH were measured using multiplex beads immunoassay. We evaluated the correlation among AqH cytokine levels, CCT, CCV4mm, and CCV6mm in eyes with bullous keratopathy. Results: The levels of protein, ILs-4, -6, -8, -10, -12p70, and -17A, MCP-1, IFN-γ E-selectin, P-selectin, and sICAM-1 were significantly higher in eyes with bullous keratopathy compared with those of the normal control subjects (all P <.0025). CCT was significantly correlated with the levels of IL-13 (r = 0.551, P =.0009) and sICAM-1 (r = 0.448, P =.0005). CCV4mm was significantly correlated with the levels of IL-13 (r = 0.514, P =.0022) and sICAM-1 (r = 0.404, P =.0019). CCV6mm was significantly correlated with the level of sICAM-1 (r = 0.459, P =.0003). Conclusion: The severity of corneal edema in eyes with bullous keratopathy was associated with the levels of specific cytokines in the AqH.
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U2 - 10.1016/j.ajo.2018.10.008
DO - 10.1016/j.ajo.2018.10.008
M3 - Article
C2 - 30316668
AN - SCOPUS:85058044134
SN - 0002-9394
VL - 198
SP - 174
EP - 180
JO - American Journal of Ophthalmology
JF - American Journal of Ophthalmology
ER -