TY - JOUR
T1 - Deamino-hydroxy-phoslactomycin B, a biosynthetic precursor of phoslactomycin, induces myeloid differentiation in HL-60 cells
AU - Simizu, Siro
AU - Teruya, Takayuki
AU - Nogawa, Toshihiko
AU - Aono, Harumi
AU - Ueki, Masashi
AU - Uramoto, Masakazu
AU - Kobayashi, Yuichi
AU - Osada, Hiroyuki
N1 - Funding Information:
The authors thank M. Ubukata, Y. Sekiyama, S. Takahashi, D. Morisaki, A. Okano, N. S. Lai, and E. Ong for excellent advice. This study was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT).
PY - 2009/6/12
Y1 - 2009/6/12
N2 - During the screening for novel differentiation inducers, we found that a culture broth of Streptomyces sp. HK-803 induced myeloid differentiation of HL-60 cells. The active substance was identified as deamino-hydroxy-phoslactomycin B (HPLM) by mass spectrometry, and synthesized HPLM also induced the differentiation of HL-60 cells. HPLM showed greater inhibition of protein phosphatase 2A (PP2A) activity than phoslactomycin B (PLMB); however, PLMB and okadaic acid did not induce differentiation. Moreover, treatment with ATRA and 1α, 25(OH)2D3 induced retinoic acid receptor-β and 1α, 25(OH)2 D3 24-hydroxylase, respectively, whereas HPLM did not, suggesting that HPLM is a novel differentiation inducer.
AB - During the screening for novel differentiation inducers, we found that a culture broth of Streptomyces sp. HK-803 induced myeloid differentiation of HL-60 cells. The active substance was identified as deamino-hydroxy-phoslactomycin B (HPLM) by mass spectrometry, and synthesized HPLM also induced the differentiation of HL-60 cells. HPLM showed greater inhibition of protein phosphatase 2A (PP2A) activity than phoslactomycin B (PLMB); however, PLMB and okadaic acid did not induce differentiation. Moreover, treatment with ATRA and 1α, 25(OH)2D3 induced retinoic acid receptor-β and 1α, 25(OH)2 D3 24-hydroxylase, respectively, whereas HPLM did not, suggesting that HPLM is a novel differentiation inducer.
KW - Acute myeloid leukemia
KW - All-trans retinoic acid
KW - Cell cycle
KW - Differentiation
KW - HL-60
KW - Natural product
KW - Phoslactomycin
KW - Protein phosphatase 2A
KW - Vitamin D
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U2 - 10.1016/j.bbrc.2009.04.014
DO - 10.1016/j.bbrc.2009.04.014
M3 - Article
C2 - 19364501
AN - SCOPUS:67349212875
SN - 0006-291X
VL - 383
SP - 406
EP - 410
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -