Decarboxylation and Racemization of Unnatural Compounds Using Artificial Enzymes Derived from Arylmalonate Decarboxylase

Kenji Miyamoto

doi:10.1002/9781118828083.ch3

Decarboxylation and Racemization of Unnatural Compounds Using Artificial Enzymes Derived from Arylmalonate Decarboxylase

Kenji Miyamoto

Department of Biosciences and Informatics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Protein engineering has emerged as a powerful tool for engineering enzymes with new functions. We have been investigating an arylmalonate decarboxylase that acts on unnatural arylmalonates to give (R)-α-arylpropionates (profens). This chapter evaluates the potential use of directed evolution and rational design in completely changing the function of the decarboxylase to an artificial racemase and (S)-selective decarboxylase. The variants are useful biocatalysts for producing optically active profens.

Original language	English
Title of host publication	Green Biocatalysis
Publisher	wiley
Pages	59-70
Number of pages	12
ISBN (Electronic)	9781118828083
ISBN (Print)	9781118822296
DOIs	https://doi.org/10.1002/9781118828083.ch3
Publication status	Published - 2016 May 13

Keywords

Artificial enzyme
Arylmalonate decarboxylase
Directed evolution
Profen

ASJC Scopus subject areas

Engineering(all)
Chemical Engineering(all)

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10.1002/9781118828083.ch3

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KW - Arylmalonate decarboxylase

KW - Directed evolution

KW - Profen

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ER -

Decarboxylation and Racemization of Unnatural Compounds Using Artificial Enzymes Derived from Arylmalonate Decarboxylase

Abstract

Keywords

ASJC Scopus subject areas

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