Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells

Yasumasa Hara; Midori A. Arai; Kanae Sakai; Naoki Ishikawa; Tohru Gonoi; Takashi Yaguchi; Masami Ishibashi

doi:10.1007/s11418-017-1161-y

Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of animal cells

Yasumasa Hara, Midori A. Arai, Kanae Sakai, Naoki Ishikawa, Tohru Gonoi, Takashi Yaguchi, Masami Ishibashi

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.

Original language	English
Pages (from-to)	280-289
Number of pages	10
Journal	Journal of Natural Medicines
Volume	72
Issue number	1
DOIs	https://doi.org/10.1007/s11418-017-1161-y
Publication status	Published - 2018 Jan 1
Externally published	Yes

Keywords

Actinomycetes
Amide
Co-culture
Nocardia

ASJC Scopus subject areas

Molecular Medicine

Access to Document

10.1007/s11418-017-1161-y

Cite this

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title = "Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells",

abstract = "A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.",

keywords = "Actinomycetes, Amide, Co-culture, Nocardia",

author = "Yasumasa Hara and Arai, {Midori A.} and Kanae Sakai and Naoki Ishikawa and Tohru Gonoi and Takashi Yaguchi and Masami Ishibashi",

note = "Funding Information: Acknowledgments This study was supported by JSPS KAKENHI Grant Number 17H03992 and the Strategic Priority Research Promotion Program of Chiba University “Phytochemical Plant Molecular Sciences”. Publisher Copyright: {\textcopyright} 2017, The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature.",

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AU - Hara, Yasumasa

AU - Arai, Midori A.

AU - Sakai, Kanae

AU - Ishikawa, Naoki

AU - Gonoi, Tohru

AU - Yaguchi, Takashi

AU - Ishibashi, Masami

N1 - Funding Information: Acknowledgments This study was supported by JSPS KAKENHI Grant Number 17H03992 and the Strategic Priority Research Promotion Program of Chiba University “Phytochemical Plant Molecular Sciences”. Publisher Copyright: © 2017, The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature.

PY - 2018/1/1

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N2 - A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.

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KW - Actinomycetes

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KW - Co-culture

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