Deletion mapping on chromosome 1p in well-differentiated gastric cancer

T. Ezaki; A. Yanagisawa; K. Ohta; S. Aiso; M. Watanabe; T. Hibi; Y. Kato; T. Nakajima; T. Ariyama; J. Inazawa; Y. Nakamura; A. Horii

doi:10.1038/bjc.1996.76

Deletion mapping on chromosome 1p in well-differentiated gastric cancer

T. Ezaki, A. Yanagisawa, K. Ohta, S. Aiso, M. Watanabe, T. Hibi, Y. Kato, T. Nakajima, T. Ariyama, J. Inazawa, Y. Nakamura, A. Horii

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Abstract

To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, D1S201 and D1S197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to 1p35 and 1p34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing these two chromosomal bands.

Original language	English
Pages (from-to)	424-428
Number of pages	5
Journal	British Journal of Cancer
Volume	73
Issue number	4
DOIs	https://doi.org/10.1038/bjc.1996.76
Publication status	Published - 1996

Keywords

Chromosome 1p
Human gastric cancer
LOH

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/bjc.1996.76

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title = "Deletion mapping on chromosome 1p in well-differentiated gastric cancer",

abstract = "To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, D1S201 and D1S197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to 1p35 and 1p34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing these two chromosomal bands.",

keywords = "Chromosome 1p, Human gastric cancer, LOH",

author = "T. Ezaki and A. Yanagisawa and K. Ohta and S. Aiso and M. Watanabe and T. Hibi and Y. Kato and T. Nakajima and T. Ariyama and J. Inazawa and Y. Nakamura and A. Horii",

note = "Funding Information: Acknowledgements The authors thank Kiyoshi Noguchi for technical assistance and Drs Yoichi Furukawa and Hirofumi Arakawa for helpful advice. This work was supported in part by the Ministry of Education, Culture and Science and the Ministry of Health and Welfare of Japan.",

year = "1996",

doi = "10.1038/bjc.1996.76",

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pages = "424--428",

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T1 - Deletion mapping on chromosome 1p in well-differentiated gastric cancer

AU - Ezaki, T.

AU - Yanagisawa, A.

AU - Ohta, K.

AU - Aiso, S.

AU - Watanabe, M.

AU - Hibi, T.

AU - Kato, Y.

AU - Nakajima, T.

AU - Ariyama, T.

AU - Inazawa, J.

AU - Nakamura, Y.

AU - Horii, A.

N1 - Funding Information: Acknowledgements The authors thank Kiyoshi Noguchi for technical assistance and Drs Yoichi Furukawa and Hirofumi Arakawa for helpful advice. This work was supported in part by the Ministry of Education, Culture and Science and the Ministry of Health and Welfare of Japan.

PY - 1996

Y1 - 1996

N2 - To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, D1S201 and D1S197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to 1p35 and 1p34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing these two chromosomal bands.

AB - To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, D1S201 and D1S197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to 1p35 and 1p34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing these two chromosomal bands.

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Deletion mapping on chromosome 1p in well-differentiated gastric cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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