Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture

Aya Mizutani Akimoto, Erika Hasuike, Hiroto Tada, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.

Original languageEnglish
Pages (from-to)1203-1206
Number of pages4
Journalanalytical sciences
Issue number11
Publication statusPublished - 2016


  • 3D cell culture
  • Hydrogel
  • Poly(N-isopropylacrylamide)
  • Thermoresponsive gel

ASJC Scopus subject areas

  • Analytical Chemistry


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