Abstract
Background: To develop a supplementary diagnostic method for endometrial cancer by measuring the reactivity of various endometrial lesions with two monoclonal antibodies. Methods: We investigated the reactivity of various endometrial lesions with two monoclonal antibodies (MSN-1 and MSN-3) by flow cytometry (one-color and two-color methods). Results: The two-color method appeared to be suitable for use in place of simultaneous performance of the one-color methods with MSN-1 and MSN-3. The positivity rate for normal endometrium was 16.0% with the two-color method, which was lower than the rate of 30.0% obtained with concomitant used of the one-color methods. The positivity rate for endometrial cancer was high, 84.0%, with the two-color method. The positivity rate was 85.7% for well-differentiated endometrial cancer, 71.4% for moderately differentiated cancer, and 100.0% for poorly differentiated cancer; thus, the rate was high irrespective of the cellular differentiation. Conclusions: The two-color method is more useful than the one-color method as a supplementary diagnostic procedure for endometrial cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 150-156 |
| Number of pages | 7 |
| Journal | Cytometry |
| Volume | 36 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 Jun 1 |
Keywords
- Diagnostic method
- Endometrial cancer
- Flow cytometry
- MSN-1
- MSN-3
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Biophysics
- Hematology
- Endocrinology
- Cell Biology
