TY - JOUR
T1 - Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction
AU - Tachibana, H.
AU - Kobayashi, S.
AU - Okuzawa, E.
AU - Masuda, G.
N1 - Funding Information:
Acknowledgements-We are grateful to Professors Y. Kaneda and T. Takeuchi for their encouragement of this study. We also thank Dr W. Stahl for reviewing the manuscript. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan; a Grant-in-Aid for a DNA diagnosis project of the Tokai University School of Medicine; a Tokai University School of Medicine Research Aid; and by a grant from the Ohyama Health Foundation to H. T.
PY - 1992/12
Y1 - 1992/12
N2 - Tachibana H., Kobayashi S., Okuzawa E. and Masuda G. 1992. Detection of pathogenic Entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction. International Journal for Parasitology 22: 1193-1196. A sensitive method for detection of pathogenic Entamoeba histolytica DNA in drained fluids from liver abscess patients, using the polymerase chain reaction (PCR), has been developed. The PCR employs oligonucleotide primers specific for the gene encoding the 30 kDa molecule of pathogenic E. histolytica. Liver abscess fluids (19 samples), from 14 patients with a presumptive amebic liver abscess, were examined microscopically and by the PCR method. Only two of the 19 samples were positive microscopically, whereas all 19 samples tested positive by PCR. This technique can be used to confirm the diagnosis of amebic liver abscess.
AB - Tachibana H., Kobayashi S., Okuzawa E. and Masuda G. 1992. Detection of pathogenic Entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction. International Journal for Parasitology 22: 1193-1196. A sensitive method for detection of pathogenic Entamoeba histolytica DNA in drained fluids from liver abscess patients, using the polymerase chain reaction (PCR), has been developed. The PCR employs oligonucleotide primers specific for the gene encoding the 30 kDa molecule of pathogenic E. histolytica. Liver abscess fluids (19 samples), from 14 patients with a presumptive amebic liver abscess, were examined microscopically and by the PCR method. Only two of the 19 samples were positive microscopically, whereas all 19 samples tested positive by PCR. This technique can be used to confirm the diagnosis of amebic liver abscess.
KW - DNA
KW - Entamoeba histolytica
KW - amebic liver abscess
KW - polymerase chain reaction
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U2 - 10.1016/0020-7519(92)90042-J
DO - 10.1016/0020-7519(92)90042-J
M3 - Article
C2 - 1487381
AN - SCOPUS:0027053433
SN - 0020-7519
VL - 22
SP - 1193
EP - 1196
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 8
ER -
