TY - JOUR
T1 - Detection of Treponema denticola in atherosclerotic lesions
AU - Okuda, K.
AU - Ishihara, K.
AU - Nakagawa, T.
AU - Hirayama, A.
AU - Inayama, Y.
AU - Okuda, K.
PY - 2001
Y1 - 2001
N2 - We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immnnofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.
AB - We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immnnofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.
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U2 - 10.1128/JCM.39.3.1114-1117.2001
DO - 10.1128/JCM.39.3.1114-1117.2001
M3 - Article
C2 - 11230436
AN - SCOPUS:0035094482
SN - 0095-1137
VL - 39
SP - 1114
EP - 1117
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 3
ER -