Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets

Misako Suzuki; Mitsuru Murata; Yumiko Matsubara; Toshihiro Uchida; Hiroaki Ishihara; Toshiro Shibano; Shin Ichiro Ashida; Kenji Soejima; Yasunori Okada; Yasuo Ikeda

doi:10.1016/j.bbrc.2003.11.111

Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets

Misako Suzuki, Mitsuru Murata, Yumiko Matsubara, Toshihiro Uchida, Hiroaki Ishihara, Toshiro Shibano, Shin Ichiro Ashida, Kenji Soejima, Yasunori Okada, Yasuo Ikeda

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.

Original language	English
Pages (from-to)	212-216
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	313
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2003.11.111
Publication status	Published - 2004 Jan 2
Externally published	Yes

Keywords

ADAMTS-13
Expression
Platelets
Real-time PCR
TTP
Thrombosis
vWF
vWF-CP

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2003.11.111

Cite this

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title = "Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets",

abstract = "The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.",

keywords = "ADAMTS-13, Expression, Platelets, Real-time PCR, TTP, Thrombosis, vWF, vWF-CP",

author = "Misako Suzuki and Mitsuru Murata and Yumiko Matsubara and Toshihiro Uchida and Hiroaki Ishihara and Toshiro Shibano and Ashida, {Shin Ichiro} and Kenji Soejima and Yasunori Okada and Yasuo Ikeda",

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AU - Suzuki, Misako

AU - Murata, Mitsuru

AU - Matsubara, Yumiko

AU - Uchida, Toshihiro

AU - Ishihara, Hiroaki

AU - Shibano, Toshiro

AU - Ashida, Shin Ichiro

AU - Soejima, Kenji

AU - Okada, Yasunori

AU - Ikeda, Yasuo

PY - 2004/1/2

Y1 - 2004/1/2

N2 - The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.

AB - The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.

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KW - Expression

KW - Platelets

KW - Real-time PCR

KW - TTP

KW - Thrombosis

KW - vWF

KW - vWF-CP

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Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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