TY - JOUR
T1 - Determination of acetylcholine concentration in cerebrospinal fluid of patients with neurologic diseases
AU - Yamada, H.
AU - Otsuka, M.
AU - Fujimoto, K.
AU - Kawashima, K.
AU - Yoshida, M.
PY - 1996
Y1 - 1996
N2 - Introduction - Acetylcholine (ACh) is a well known neurotransmitter in the central nervous system, but determining its level in cerebrospinal fluid (CSF) is very difficult and the origin of CSF ACh is still unknown. In this study, we attempted to measure CSF ACh by a specific and sensitive radioimmunoassay (RIA) from patients with neurologic diseases. Material and methods - Patients with cerebral infarction (n = 7), Parkinson's disease (n = 6), spinocerebellar degeneration (n = 6), Alzheimer's disease (n = 3), amyotrophic lateral sclerosis (n = 3) and disc herniation with no central nervous involvement (n = 8) participated to determine the CSF ACh levels. Results - Of these 33 patients, the mean ACh level in CSF was 282.2 ± 61.7 fmol/ml (mean ± SE, range 20-1505.8 fmol/ml). The mean ACh level of spinocerebellar degeneration group was lower than others, but not statistically significant. Conclusion - We conclude that an amount of ACh detectable by RIA is certainly present in CSF.
AB - Introduction - Acetylcholine (ACh) is a well known neurotransmitter in the central nervous system, but determining its level in cerebrospinal fluid (CSF) is very difficult and the origin of CSF ACh is still unknown. In this study, we attempted to measure CSF ACh by a specific and sensitive radioimmunoassay (RIA) from patients with neurologic diseases. Material and methods - Patients with cerebral infarction (n = 7), Parkinson's disease (n = 6), spinocerebellar degeneration (n = 6), Alzheimer's disease (n = 3), amyotrophic lateral sclerosis (n = 3) and disc herniation with no central nervous involvement (n = 8) participated to determine the CSF ACh levels. Results - Of these 33 patients, the mean ACh level in CSF was 282.2 ± 61.7 fmol/ml (mean ± SE, range 20-1505.8 fmol/ml). The mean ACh level of spinocerebellar degeneration group was lower than others, but not statistically significant. Conclusion - We conclude that an amount of ACh detectable by RIA is certainly present in CSF.
KW - Acetylcholine
KW - Cerebrospinal fluid
KW - Neurologic disease
KW - Radioimmunoassay
UR - http://www.scopus.com/inward/record.url?scp=0030044810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030044810&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0404.1996.tb00175.x
DO - 10.1111/j.1600-0404.1996.tb00175.x
M3 - Article
C2 - 8825278
AN - SCOPUS:0030044810
SN - 0001-6314
VL - 93
SP - 76
EP - 78
JO - Acta Neurologica Scandinavica
JF - Acta Neurologica Scandinavica
IS - 1
ER -