Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis

Makoto Tsunoda, Masaru Kato, Takeshi Fukushima, Tomofumi Santa, Hiroshi Homma, Hiroko Yanai, Tomoyoshi Soga, Kazuhiro Imai

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalBiomedical Chromatography
Issue number5
Publication statusPublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry


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