TY - JOUR
T1 - Determination of carbohydrates by hydrophilic interaction chromatography with pulsed amperometric detection using postcolumn pH adjustment
AU - Soga, Tomoyoshi
AU - Inoue, Yoshinori
AU - Yamaguchi, Kenji
PY - 1992/11/20
Y1 - 1992/11/20
N2 - An HPLC method for the determination of carbohydrates using a postcolumn pH adjustment technique with lithium hydroxide solution followed by pulsed amperometric detection was developed. In conjunction with hydrophilic interaction chromatography, the technique was used to determine reducing sugars, non-reducing sugars and sugar alcohols. Detection limits from 0.7 to 2.7 ng with a signal-to-noise ratio of 3 were achieved. The relative standard deviation for peak was better than 2.7%. The postcolumn pH adjustment technique enabled gradient elution to be used.
AB - An HPLC method for the determination of carbohydrates using a postcolumn pH adjustment technique with lithium hydroxide solution followed by pulsed amperometric detection was developed. In conjunction with hydrophilic interaction chromatography, the technique was used to determine reducing sugars, non-reducing sugars and sugar alcohols. Detection limits from 0.7 to 2.7 ng with a signal-to-noise ratio of 3 were achieved. The relative standard deviation for peak was better than 2.7%. The postcolumn pH adjustment technique enabled gradient elution to be used.
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U2 - 10.1016/0021-9673(92)85196-Z
DO - 10.1016/0021-9673(92)85196-Z
M3 - Article
AN - SCOPUS:0026496242
SN - 0021-9673
VL - 625
SP - 151
EP - 155
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -