Abstract
A simple method for the quantitative determination of erythromycin (EM) concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection was developed. EM was extracted from 200 μl of plasma or liver homogenate sample under sodium hydroxide alkaline conditions with tert.-butyl methyl ether. Oleandomycin was used as an internal standard. The recovery rate of EM was up to 100%. The detector cell potential for the oxidation of EM was +1100 mV. The calibration curves were linear over the concentration ranges 0.1-20.0 μg/ml for plasma and 0.5-100.0 μg/g for liver. The method was applied to the determination of the plasma and liver concentrations of EM in rats after intravenous administration (50 mg/kg dose). The method presented here has proved to be of great use for the investigation of the pharmacokinetic characteristics of EM in small animals such as rats.
| Original language | English |
|---|---|
| Pages (from-to) | 478-482 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 692 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1997 May 9 |
| Externally published | Yes |
Keywords
- Erythromycin
ASJC Scopus subject areas
- Chemistry(all)