Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis

Hideyuki Nishizawa; Natsumi Kita; Sakiko Okimura; Emi Takao; Yoshihiro Abe

doi:10.1002/elps.1150091203

Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis

Hideyuki Nishizawa, Natsumi Kita, Sakiko Okimura, Emi Takao, Yoshihiro Abe

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

Original language	English
Pages (from-to)	803-806
Number of pages	4
Journal	ELECTROPHORESIS
Volume	9
Issue number	12
DOIs	https://doi.org/10.1002/elps.1150091203
Publication status	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.1150091203

Cite this

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title = "Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis",

abstract = "An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.",

author = "Hideyuki Nishizawa and Natsumi Kita and Sakiko Okimura and Emi Takao and Yoshihiro Abe",

year = "1988",

doi = "10.1002/elps.1150091203",

language = "English",

volume = "9",

pages = "803--806",

journal = "ELECTROPHORESIS",

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T1 - Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis

AU - Nishizawa, Hideyuki

AU - Kita, Natsumi

AU - Okimura, Sakiko

AU - Takao, Emi

AU - Abe, Yoshihiro

PY - 1988

Y1 - 1988

N2 - An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

AB - An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

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U2 - 10.1002/elps.1150091203

DO - 10.1002/elps.1150091203

M3 - Article

C2 - 3243233

AN - SCOPUS:0024202792

SN - 0173-0835

VL - 9

SP - 803

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JO - ELECTROPHORESIS

JF - ELECTROPHORESIS

IS - 12

ER -

Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis

Abstract

ASJC Scopus subject areas

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