Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides

Noboru Fujimoto; Hideaki Tokai; Kazushi Iwata; Yasunori Okada; Taro Hayakawa

doi:10.1016/0022-1759(95)00164-6

Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides

Noboru Fujimoto, Hideaki Tokai, Kazushi Iwata, Yasunori Okada, Taro Hayakawa

Department of Pathology

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

Original language	English
Pages (from-to)	33-39
Number of pages	7
Journal	Journal of Immunological Methods
Volume	187
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(95)00164-6
Publication status	Published - 1995 Nov 16

Keywords

Enzyme immunoassay
Matrix metalloproteinase
Monoclonal antibody
Tissue inhibitor of metalloproteinases-2

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(95)00164-6

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title = "Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides",

abstract = "We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.",

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author = "Noboru Fujimoto and Hideaki Tokai and Kazushi Iwata and Yasunori Okada and Taro Hayakawa",

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AU - Fujimoto, Noboru

AU - Tokai, Hideaki

AU - Iwata, Kazushi

AU - Okada, Yasunori

AU - Hayakawa, Taro

PY - 1995/11/16

Y1 - 1995/11/16

N2 - We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

AB - We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

KW - Enzyme immunoassay

KW - Matrix metalloproteinase

KW - Monoclonal antibody

KW - Tissue inhibitor of metalloproteinases-2

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Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides

Abstract

Keywords

ASJC Scopus subject areas

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