@article{afa2ed8956a449c89428e3a90a6e75bf,
title = "Development of a sensitive bioluminogenic probe for imaging highly reactive oxygen species in living rats",
abstract = "A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO3H-APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme-induced electron transfer (BioLeT) and modulation of cell-membrane permeability. This probe enables non-invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes. You light up my life: A sensitive bioluminogenic probe for highly reactive oxygen species (hROS) was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme-induced electron transfer (BioLeT) and modulation of cell-membrane permeability. This probe enables non-invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats.",
keywords = "electron transfer, imaging agents, luminescence, membrane permeability, reactive oxygen species",
author = "Ryosuke Kojima and Hideo Takakura and Mako Kamiya and Eiji Kobayashi and Toru Komatsu and Tasuku Ueno and Takuya Terai and Kenjiro Hanaoka and Tetsuo Nagano and Yasuteru Urano",
note = "Funding Information: We thank Dr. S. Uno (Univ. Tokyo) for providing 4-COOH MMSiR, Dr. Y. Isobe and Mr. T. Kato (Univ. Tokyo) for their technical help in collecting neutrophils, and Prof. T. Ozawa (Univ. Tokyo) for the luc 2 plasmid. This research was supported in part by a Grant-in-Aid for JSPS Fellows (to R.K. and H.T.), the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant-in-Aid for Scientific Research (KAKENHI), Grants 20117003, 23249004 and 26111012 to Y.U., 25113707 and 15H05951 to M.K.), by The Daiichi-Sankyo Foundation of Life Science (grant to Y.U.), by The Mochida Memorial Foundation for Medical and Pharmaceutical Research (grant to M.K.), by JSPS Core-to-Core Program, A. Advanced Research Networks. E.K. had been the Chief Scientific Advisor for Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan from 2009 to 2014. Luciferase transgenic rats used in this study are the subject of a Japanese patent (patent number: 5198433). E.K. received honoraria and research funding from Berthold Japan K.K. Publisher Copyright: {\textcopyright} 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2015",
month = dec,
day = "1",
doi = "10.1002/anie.201507530",
language = "English",
volume = "54",
pages = "14768--14771",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "49",
}