Development of chromatography system organic solvent-free using multi-functional polymers

Tadashi Nishio, Hideko Kanazawa

Research output: Contribution to journalArticlepeer-review


We have developed a novel chromatography system, an environmental-responsive chromatography, based on a multifunctional polymer. We designed a temperature-responsive copolymer with N-isopropylacrylamide and various monomers. This copolymer undergoes a reversible phase transition from a hydrophilic to a hydrophobic microstructure when triggered by a change of temperature. Also, with this chromatographic system is possible to separate the analytes by using only an aqueous solution or water as the mobile phase. Temperature-responsive chromatography could prove to be highly useful for bioactive molecules, such as steroid, amino acid and peptide separation. There are some advantages not to need an organic solvent, compared to traditional reversed-phase chromatography. For example, we achieved a successful separation of enzymes without a loss of bioactivity, and for environmental use as a new green technology, and for reducing the cost of handling and disposing of a mobile phase. Applications to bio-separation in various fields, such as medical and pharmaceutical sciences, are expected in the future.

Original languageEnglish
Pages (from-to)163-173
Number of pages11
Issue number3
Publication statusPublished - 2010
Externally publishedYes


  • Bioactive compounds
  • Environmental-responsive chromatography
  • Green technology
  • Medical and pharmaceutical sciences
  • Poly(N-isopropylacrylamide)

ASJC Scopus subject areas

  • Analytical Chemistry


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