Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging

Yuichiro Koide; Yasuteru Urano; Kenjiro Hanaoka; Wen Piao; Moriaki Kusakabe; Nae Saito; Takuya Terai; Takayoshi Okabe; Tetsuo Nagano

doi:10.1021/ja210375e

Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging

Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Wen Piao, Moriaki Kusakabe, Nae Saito, Takuya Terai, Takayoshi Okabe, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

230 Citations (Scopus)

Abstract

We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.

Original language	English
Pages (from-to)	5029-5031
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	134
Issue number	11
DOIs	https://doi.org/10.1021/ja210375e
Publication status	Published - 2012 Mar 21
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja210375e

Cite this

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abstract = "We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.",

author = "Yuichiro Koide and Yasuteru Urano and Kenjiro Hanaoka and Wen Piao and Moriaki Kusakabe and Nae Saito and Takuya Terai and Takayoshi Okabe and Tetsuo Nagano",

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AU - Koide, Yuichiro

AU - Urano, Yasuteru

AU - Hanaoka, Kenjiro

AU - Piao, Wen

AU - Kusakabe, Moriaki

AU - Saito, Nae

AU - Terai, Takuya

AU - Okabe, Takayoshi

AU - Nagano, Tetsuo

PY - 2012/3/21

Y1 - 2012/3/21

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AB - We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.

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Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging

Abstract

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