Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation

Tetsuya Mizuno; Keitaro Umezawa; Yutaka Shindo; Daniel Citterio; Kotaro Oka; Koji Suzuki

doi:10.1007/s10895-013-1227-5

Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation

Tetsuya Mizuno, Keitaro Umezawa, Yutaka Shindo, Daniel Citterio, Kotaro Oka, Koji Suzuki

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca²⁺/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca²⁺ indicator) reveals its usefulness as a new bioimaging tool.

Original language	English
Pages (from-to)	1007-1018
Number of pages	12
Journal	Journal of Fluorescence
Volume	23
Issue number	5
DOIs	https://doi.org/10.1007/s10895-013-1227-5
Publication status	Published - 2013 Sept

Keywords

BODIPY
Bioimaging
Fluorescent dyads
Near-infrared fluorophore

ASJC Scopus subject areas

Biochemistry
Spectroscopy
Clinical Biochemistry

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10.1007/s10895-013-1227-5

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title = "Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation",

abstract = "We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.",

keywords = "BODIPY, Bioimaging, Fluorescent dyads, Near-infrared fluorophore",

author = "Tetsuya Mizuno and Keitaro Umezawa and Yutaka Shindo and Daniel Citterio and Kotaro Oka and Koji Suzuki",

note = "Funding Information: Acknowledgement We acknowledge T. Shichi (Nissan Arc. Ltd.) for his help with ESI-MS data acquisition, and Y. Urano (The University of Tokyo) for his support for fluorescence lifetime measurements. This work was supported by a JSPS stipend to K.U. and a Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 20245019).",

year = "2013",

month = sep,

doi = "10.1007/s10895-013-1227-5",

language = "English",

volume = "23",

pages = "1007--1018",

journal = "Journal of Fluorescence",

issn = "1053-0509",

publisher = "Springer New York",

number = "5",

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TY - JOUR

T1 - Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation

AU - Mizuno, Tetsuya

AU - Umezawa, Keitaro

AU - Shindo, Yutaka

AU - Citterio, Daniel

AU - Oka, Kotaro

AU - Suzuki, Koji

N1 - Funding Information: Acknowledgement We acknowledge T. Shichi (Nissan Arc. Ltd.) for his help with ESI-MS data acquisition, and Y. Urano (The University of Tokyo) for his support for fluorescence lifetime measurements. This work was supported by a JSPS stipend to K.U. and a Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 20245019).

PY - 2013/9

Y1 - 2013/9

N2 - We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.

AB - We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.

KW - BODIPY

KW - Bioimaging

KW - Fluorescent dyads

KW - Near-infrared fluorophore

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DO - 10.1007/s10895-013-1227-5

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SN - 1053-0509

VL - 23

SP - 1007

EP - 1018

JO - Journal of Fluorescence

JF - Journal of Fluorescence

IS - 5

ER -

Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation

Abstract

Keywords

ASJC Scopus subject areas

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