TY - JOUR
T1 - Developmental expression of the inositol 1,4,5-trisphosphate receptor and structural changes in the endoplasmic reticulum during oogenesis and meiotic maturation of Xenopus laevis
AU - Kume, Shoen
AU - Yamamoto, Akitsugu
AU - Inoue, Takafumi
AU - Muto, Akira
AU - Okano, Hideyuki
AU - Mikoshiba, Katsuhiko
N1 - Funding Information:
We thank Dr. Y. Tashiro (Department of Physiology, Kansai Medical University) for invaluable discussions, Dr. Kunio Kato for advise on computer statistical analyses, and Kei Suga and Keiko Okabe for technical assistance. This work was supported by grants from the Japanese Ministry of Education, Science, Sports and Culture to H.O. and K.M. and grants from Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists to S.K.
PY - 1997/2/15
Y1 - 1997/2/15
N2 - To study the development of the calcium release mechanism, we examined the temporal and spatial expression of inositol 1,4,5-trisphosphate receptor (IP3R) during the oogenesis and meiotic maturation of Xenopus laevis. Observation of a series of fixed samples by immunofluorescence microscopy revealed a relocalization of Xenopus IP3R (XIP3R)-positive structures during meiotic maturation. We visualized the endoplasmic reticulum (ER) using ER-sensitive dye, DiI, by observation under confocal laser scanning microscopy. Time-lapse visualization of the living oocytes revealed that while the ER of immature fully grown oocytes underwent relatively little movement, the ER of maturing oocytes and mature eggs moved rapidly. A possible role for the increase of ER mobility in the dynamic redistribution of XIP3R during oocyte maturation is also discussed.
AB - To study the development of the calcium release mechanism, we examined the temporal and spatial expression of inositol 1,4,5-trisphosphate receptor (IP3R) during the oogenesis and meiotic maturation of Xenopus laevis. Observation of a series of fixed samples by immunofluorescence microscopy revealed a relocalization of Xenopus IP3R (XIP3R)-positive structures during meiotic maturation. We visualized the endoplasmic reticulum (ER) using ER-sensitive dye, DiI, by observation under confocal laser scanning microscopy. Time-lapse visualization of the living oocytes revealed that while the ER of immature fully grown oocytes underwent relatively little movement, the ER of maturing oocytes and mature eggs moved rapidly. A possible role for the increase of ER mobility in the dynamic redistribution of XIP3R during oocyte maturation is also discussed.
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U2 - 10.1006/dbio.1996.8479
DO - 10.1006/dbio.1996.8479
M3 - Article
C2 - 9070324
AN - SCOPUS:0031568817
SN - 0012-1606
VL - 182
SP - 228
EP - 239
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -