TY - JOUR
T1 - Developmental expression of the inositol 1,4,5-trisphosphate receptor and localization of inositol 1,4,5-trisphosphate during early embryogenesis in Xenopus laevis
AU - Kume, Shoen
AU - Muto, Akira
AU - Okano, Hideyuki
AU - Mikoshiba, Katsuhiko
N1 - Funding Information:
We thank Dr. M. Asashima for invaluable discussions,K . Okabe for technical assistance,M . Ohara for critical readings of the manuscript.T his work was supportedi n part by grants from the Japanese Ministry of Education, Science, Sports and Culture to H.O. and K.M. and grants from Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (to S.K.).
PY - 1997/8
Y1 - 1997/8
N2 - To study the role of inositol 1,4,5-trisphosphate (IP3) receptors during early embryogenesis in Xenopus, we examined the temporal-spatial localization of Xenopus IP3 receptor (XIP3R), XIP3R protein is enriched in the animal hemisphere of early cleavage stage embryos and becomes localized in the ectoderm and involuted mesoderm in gastrula stage embryos. Up to tailbud stages, expression of XIP3R is observed in the mesodermal tissues and in most subregions of the central nervous system. A quantitative analysis of endogenous IP3 mass during normal early embryogenesis revealed an increase in IP3 mass first observed at early gastrula stage of 10.5 with an enrichment in the ectoderm throughout the gastrula stages, implying a potential role during gastrulation.
AB - To study the role of inositol 1,4,5-trisphosphate (IP3) receptors during early embryogenesis in Xenopus, we examined the temporal-spatial localization of Xenopus IP3 receptor (XIP3R), XIP3R protein is enriched in the animal hemisphere of early cleavage stage embryos and becomes localized in the ectoderm and involuted mesoderm in gastrula stage embryos. Up to tailbud stages, expression of XIP3R is observed in the mesodermal tissues and in most subregions of the central nervous system. A quantitative analysis of endogenous IP3 mass during normal early embryogenesis revealed an increase in IP3 mass first observed at early gastrula stage of 10.5 with an enrichment in the ectoderm throughout the gastrula stages, implying a potential role during gastrulation.
KW - IP
KW - IP receptor
KW - Localization
KW - Xenopus
UR - http://www.scopus.com/inward/record.url?scp=0030929042&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030929042&partnerID=8YFLogxK
U2 - 10.1016/S0925-4773(97)00101-9
DO - 10.1016/S0925-4773(97)00101-9
M3 - Article
C2 - 9376319
AN - SCOPUS:0030929042
SN - 0925-4773
VL - 66
SP - 157
EP - 168
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 1-2
ER -