TY - JOUR
T1 - Developments and applications of mass microscopy
AU - Setou, Mitsutoshi
AU - Shrivas, Kamlesh
AU - Sroyraya, Morakot
AU - Yang, Hyunjeong
AU - Sugiura, Yuki
AU - Moribe, Junji
AU - Kondo, Akira
AU - Tsutsumi, Koji
AU - Kimura, Yoshishige
AU - Kurabe, Nobuya
AU - Hayasaka, Takahiro
AU - Goto-Inoue, Naoko
AU - Zaima, Nobuhiro
AU - Ikegami, Koji
AU - Sobhon, Prasert
AU - Konishi, Yoshiyuki
PY - 2010/3
Y1 - 2010/3
N2 - We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrixassisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution of biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections. Mass microscopy provides clear images about the distribution of hundreds of biomolecules in a single measurement and also helps in understanding the cellular profile of the biological system. The sample preparation and the spatial resolution and speed of the technique are all important steps that affect the identification of biomolecules in mass microscopy. In this Award Lecture Review, we focus on some of the recent developments in clinical applications to show how mass microscopy can be employed to assess medical molecular morphology.
AB - We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrixassisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution of biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections. Mass microscopy provides clear images about the distribution of hundreds of biomolecules in a single measurement and also helps in understanding the cellular profile of the biological system. The sample preparation and the spatial resolution and speed of the technique are all important steps that affect the identification of biomolecules in mass microscopy. In this Award Lecture Review, we focus on some of the recent developments in clinical applications to show how mass microscopy can be employed to assess medical molecular morphology.
KW - Biomolecules
KW - Clinical samples
KW - Imaging mass spectrometry
KW - Mass microscope
KW - Matrix-assisted laser desorption/ionization (MALDI)
KW - Nanoparticle-based laser desorption/ionization (nano-PALDI)
UR - http://www.scopus.com/inward/record.url?scp=77954613244&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954613244&partnerID=8YFLogxK
U2 - 10.1007/s00795-009-0489-0
DO - 10.1007/s00795-009-0489-0
M3 - Review article
C2 - 20339999
AN - SCOPUS:77954613244
SN - 1860-1480
VL - 43
SP - 1
EP - 5
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
IS - 1
ER -