TY - JOUR
T1 - Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers
AU - Susumu, Nobuyuki
AU - Aoki, D.
AU - Noda, T.
AU - Nagashima, Y.
AU - Hirao, T.
AU - Tamada, Y.
AU - Banno, K.
AU - Suzuki, A.
AU - Suzuki, N.
AU - Tsuda, H.
AU - Inazawa, J.
AU - Nozawa, S.
PY - 2005/1/1
Y1 - 2005/1/1
N2 - We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI17-321) and 17p13.3 (D17S34), and a combination of the probes designed for Iq12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95% (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93% (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90%) and five (100%) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.
AB - We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI17-321) and 17p13.3 (D17S34), and a combination of the probes designed for Iq12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95% (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93% (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90%) and five (100%) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.
KW - Cytological diagnosis
KW - Endometrial cancer
KW - Liquid-based cytology
KW - Two-color FISH
UR - http://www.scopus.com/inward/record.url?scp=19944430220&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19944430220&partnerID=8YFLogxK
U2 - 10.1111/j.1048-891x.2005.14424.x
DO - 10.1111/j.1048-891x.2005.14424.x
M3 - Article
C2 - 15670300
AN - SCOPUS:19944430220
SN - 1048-891X
VL - 15
SP - 70
EP - 80
JO - International Journal of Gynecological Cancer
JF - International Journal of Gynecological Cancer
IS - 1
ER -
