TY - GEN
T1 - Dielectrophoretic sorting system for screening of NAD(P)-dependent oxidoreductases.
AU - Kanai, Yuki
AU - Shimokihara, Shota
AU - Shitara, Shunya
AU - Oyobiki, Ryo
AU - Watanabe, Takeshi
AU - Einaga, Yasuaki
AU - Wakamatsu, Kunihiko
AU - Matsumoto, Yoshinori
AU - Fujiwara, Kei
AU - Doi, Nobuhide
N1 - Funding Information:
We thank members of our laboratory for experimental advice and useful discussions. This work was supported by a Grant-in-Aid for Scientific Research (24656508) from the JSPS of Japan, a Keio Gijyuku Academic Development Fund, and the Asahi Glass Foundation.
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - Here we report the development of a flow injection analysis system based on electrochemical measurements and dielectrophoresis for screening of NAD(P)-dependent oxidoreductases. In this system, the activity of enzyme which is immobilized on microbeads together with its encoding DNA can be measured with a boron-doped diamond (BDD) electrode in each compartment (~30 nL) of the microfluidic system, and the large droplets (~30 nL) containing genes of enzymes with higher activity can be recovered by dielectrophoretic sorting.
AB - Here we report the development of a flow injection analysis system based on electrochemical measurements and dielectrophoresis for screening of NAD(P)-dependent oxidoreductases. In this system, the activity of enzyme which is immobilized on microbeads together with its encoding DNA can be measured with a boron-doped diamond (BDD) electrode in each compartment (~30 nL) of the microfluidic system, and the large droplets (~30 nL) containing genes of enzymes with higher activity can be recovered by dielectrophoretic sorting.
KW - Boron-doped diamond electrode
KW - Cell-free protein synthesis
KW - Dielectrophoresis
KW - Directed evolution
KW - High-throughput screening
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M3 - Conference contribution
AN - SCOPUS:85079618432
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 1383
EP - 1384
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -