Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines

Kazuhiro Atsukawa; Hidetsugu Saito; Masahiko Takahashi; Yasutada Akiba; Tazuko Ohishi; Hirotoshi Ebinuma; Iwao Kurose; Hiromasa Ishii

doi:10.1016/S0928-4346(97)00365-4

Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines

Kazuhiro Atsukawa, Hidetsugu Saito, Masahiko Takahashi, Yasutada Akiba, Tazuko Ohishi, Hirotoshi Ebinuma, Iwao Kurose, Hiromasa Ishii

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Effects of interleukin-4 (IL-4) and interferon-γ (IFN-γ) on rat Kupffer cell (KC) functions were investigated. Lipopolysaccharide (LPS)-stimulated KCs pretreated with IL-4 produced much more prostaglandin E₂ than those pretreated with IFN-γ. In contrast, LPS-stimulated KCs pretreated with IFN-γ produced much more tumor necrosis factor-α, and nitrate and nitrite than those pretreated with IL-4. Different morphologic changes were induced after the culture with each cytokine; transformation of KCs into multinucleated giant cells with IL-4 was especially noted. These results suggest that IFN-γ and IL-4, produced by different subsets of T-helper lymphocytes, differently induce KC into specific morphology, and change KC response to endotoxins, possibly resulting in a modulation of hepatic inflammation.

Original language	English
Pages (from-to)	332-338
Number of pages	7
Journal	International Hepatology Communications
Volume	6
Issue number	6
DOIs	https://doi.org/10.1016/S0928-4346(97)00365-4
Publication status	Published - 1997 Mar
Externally published	Yes

Keywords

interferon-γ
interleukin-4
nitric oxide
prostaglandin E
tumor necrosis factor-α

ASJC Scopus subject areas

Hepatology

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10.1016/S0928-4346(97)00365-4

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@article{bbd17f1166234fd89cd62ba907bfcf2b,

title = "Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines",

abstract = "Effects of interleukin-4 (IL-4) and interferon-γ (IFN-γ) on rat Kupffer cell (KC) functions were investigated. Lipopolysaccharide (LPS)-stimulated KCs pretreated with IL-4 produced much more prostaglandin E2 than those pretreated with IFN-γ. In contrast, LPS-stimulated KCs pretreated with IFN-γ produced much more tumor necrosis factor-α, and nitrate and nitrite than those pretreated with IL-4. Different morphologic changes were induced after the culture with each cytokine; transformation of KCs into multinucleated giant cells with IL-4 was especially noted. These results suggest that IFN-γ and IL-4, produced by different subsets of T-helper lymphocytes, differently induce KC into specific morphology, and change KC response to endotoxins, possibly resulting in a modulation of hepatic inflammation.",

keywords = "interferon-γ, interleukin-4, nitric oxide, prostaglandin E, tumor necrosis factor-α",

author = "Kazuhiro Atsukawa and Hidetsugu Saito and Masahiko Takahashi and Yasutada Akiba and Tazuko Ohishi and Hirotoshi Ebinuma and Iwao Kurose and Hiromasa Ishii",

note = "Funding Information: The authors would like to thank MS Ruri C. Nakatsumi for her technical assistance. Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and by a grant from Keio University, School of Medicine.",

year = "1997",

month = mar,

doi = "10.1016/S0928-4346(97)00365-4",

language = "English",

volume = "6",

pages = "332--338",

journal = "International Hepatology Communications",

issn = "0928-4346",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "6",

}

TY - JOUR

T1 - Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines

AU - Atsukawa, Kazuhiro

AU - Saito, Hidetsugu

AU - Takahashi, Masahiko

AU - Akiba, Yasutada

AU - Ohishi, Tazuko

AU - Ebinuma, Hirotoshi

AU - Kurose, Iwao

AU - Ishii, Hiromasa

N1 - Funding Information: The authors would like to thank MS Ruri C. Nakatsumi for her technical assistance. Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and by a grant from Keio University, School of Medicine.

PY - 1997/3

Y1 - 1997/3

N2 - Effects of interleukin-4 (IL-4) and interferon-γ (IFN-γ) on rat Kupffer cell (KC) functions were investigated. Lipopolysaccharide (LPS)-stimulated KCs pretreated with IL-4 produced much more prostaglandin E2 than those pretreated with IFN-γ. In contrast, LPS-stimulated KCs pretreated with IFN-γ produced much more tumor necrosis factor-α, and nitrate and nitrite than those pretreated with IL-4. Different morphologic changes were induced after the culture with each cytokine; transformation of KCs into multinucleated giant cells with IL-4 was especially noted. These results suggest that IFN-γ and IL-4, produced by different subsets of T-helper lymphocytes, differently induce KC into specific morphology, and change KC response to endotoxins, possibly resulting in a modulation of hepatic inflammation.

AB - Effects of interleukin-4 (IL-4) and interferon-γ (IFN-γ) on rat Kupffer cell (KC) functions were investigated. Lipopolysaccharide (LPS)-stimulated KCs pretreated with IL-4 produced much more prostaglandin E2 than those pretreated with IFN-γ. In contrast, LPS-stimulated KCs pretreated with IFN-γ produced much more tumor necrosis factor-α, and nitrate and nitrite than those pretreated with IL-4. Different morphologic changes were induced after the culture with each cytokine; transformation of KCs into multinucleated giant cells with IL-4 was especially noted. These results suggest that IFN-γ and IL-4, produced by different subsets of T-helper lymphocytes, differently induce KC into specific morphology, and change KC response to endotoxins, possibly resulting in a modulation of hepatic inflammation.

KW - interferon-γ

KW - interleukin-4

KW - nitric oxide

KW - prostaglandin E

KW - tumor necrosis factor-α

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DO - 10.1016/S0928-4346(97)00365-4

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JO - International Hepatology Communications

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ER -

Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines

Abstract

Keywords

ASJC Scopus subject areas

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