@article{bac5b2c6732740ed8159d0fdfbe5e15f,
title = "Differential ability of polymorphic OGG1 proteins to suppress mutagenesis induced by 8-hydroxyguanine in human cell in vivo",
abstract = "OGG1 protein has an ability to suppress mutagenesis induced by 8-hydroxyguanine (8OHG), an oxidatively damaged promutagenic base. Here, the mutation suppressive ability was compared between two common polymorphic OGG1 proteins, OGG1-Ser326 and OGG1-Cys326, using a supF forward mutation assay employing an 8OHG-containing plasmid. Polymorphic OGG1 proteins were exogenously expressed by adenoviral transduction in H1299 human lung cancer cells, in which endogenous OGG1 protein was undetectable by western blot analysis. Mutations by 8OHG were more efficiently suppressed in OGG1-Ser326 transduced cells than OGG1-Cys326 transduced cells. The results indicated that OGG1-Cys326 has a lower ability to prevent mutagenesis by 8OHG than OGG1-Ser326 in vivo in human cells; supporting the results of recent association studies that OGG1-Cys326 is a risk allele for several types of human cancers.",
author = "Arito Yamane and Takashi Kohno and Kohei Ito and Noriaki Sunaga and Kazunori Aoki and Kimio Yoshimura and Hirokazu Murakami and Yoshihisa Nojima and Jun Yokota",
note = "Funding Information: The authors would like to thank Dr J.D.Minna and Dr A.F.Gazdar of the University of Texas Southwestern Medical Center, USA, for providing us with the H1299 cell line, and Dr T.Matsuda of the Hall of Global Environmental Research, Graduate School of Global Environmental Studies, Kyoto University, Japan, for providing us with the pMY189 plasmid. This work was supported by Grants-in-Aid from the Ministry of Health, Labour and Welfare, from the Ministry of Education, Culture, Sports, Science and Technology for Scientific Research on Priority Areas (KAKENHI 15023265 and 16021264) and from the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research. N.S. was awarded a Research Resident Fellowship from the Foundation for Promotion of Cancer Research in Japan during the period of this study.",
year = "2004",
month = sep,
doi = "10.1093/carcin/bgh166",
language = "English",
volume = "25",
pages = "1689--1694",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "9",
}
