Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation

K. Asano, M. Nakamura, T. Oguma, K. Fukunaga, H. Matsubara, T. Shiomi, A. Ishizaka, K. Yamaguchi, M. Kanazawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Objective and design: The gene expression profile of CCR3 ligands, eotaxin, RANTES, and monocyte chemo-attractant protein-3 (MCP-3), was examined in normal and inflamed guinea pig lungs. Material: Male Hartley guinea pigs (n = 49). Methods: Pulmonary mRNA was obtained from naive animals, animals treated with intravenous lipopolysaccharide administration, and animals repeatedly exposed to aerosolized allergen (ovalbumin). Northern analysis was performed to quantify pulmonary expression of eotaxin, RANTES, and MCP-3 mRNA. Pulmonary eosinophil peroxidase (EPO) activity was measured to quantify eosinophil accumulation. Results: Eotaxin and RANTES mRNAs, but not MCP-3 mRNA, were constitutively expressed in guinea pig lungs. Lipopolysaccharide treatment increased MCP-3 mRNA expression, but not eotaxin or RANTES mRNA. In contrast, allergen exposure in sensitized animals caused an increase in eotaxin mRNA, which demonstrated good temporal and quantitative correlation with pulmonary EPa activity, but not in MCP-3 or RANTES mRNA. Conclusions: Guinea pig CCR3 ligands demonstrated different gene expression profiles in normal and inflamed airways, suggesting that they play different physiological and pathophysiological roles in the airway.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalInflammation Research
Issue number12
Publication statusPublished - 2001


  • Asthma
  • Eosinophil
  • Eotaxin
  • Monocyte chemoattractant protein

ASJC Scopus subject areas

  • Immunology
  • Pharmacology


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