Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

Satoshi Baba, Ayumi Taguchi, Akira Kawata, Konan Hara, Satoko Eguchi, Mayuyo Mori, Katsuyuki Adachi, Seiichiro Mori, Takashi Iwata, Akira Mitsuhashi, Daichi Maeda, Atsushi Komatsu, Takeshi Nagamatsu, Katsutoshi Oda, Iwao Kukimoto, Yutaka Osuga, Tomoyuki Fujii, Kei Kawana

Research output: Contribution to journalArticlepeer-review


Background: Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. Methods: In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6*, E1^E4, and viral capsid protein L1 in four major HPV genotypes-HPV 16, 18, 52, and 58-were investigated. Results: The detection rate of E6/E6*increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1. Conclusions: We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis.

Original languageEnglish
Article number32
JournalVirology Journal
Issue number1
Publication statusPublished - 2020 Mar 6


  • Cervical intraepithelial neoplasia
  • Human papillomavirus
  • Viral transcripts

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases


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