TY - GEN
T1 - Differentiation of neural stem cells regulated by three-dimensional tissue shape
AU - Matsushiro, Yuki
AU - Kato-Negishi, Midori
AU - Onoe, Hiroaki
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Challenging Exploratory Research (No. 25600055) from Japan Society of the Promotion of Science (JSPS), Japan.
Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/26
Y1 - 2017/7/26
N2 - This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.
AB - This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.
KW - Agarose hydrogel
KW - Differentiation induction
KW - Micropatterning
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=85029388133&partnerID=8YFLogxK
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U2 - 10.1109/TRANSDUCERS.2017.7994044
DO - 10.1109/TRANSDUCERS.2017.7994044
M3 - Conference contribution
AN - SCOPUS:85029388133
T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 285
EP - 288
BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Y2 - 18 June 2017 through 22 June 2017
ER -