Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Yohei Bamba; Tomoko Shofuda; Daisuke Kanematsu; Masahiro Nonaka; Mami Yamasaki; Hideyuki Okano; Yonehiro Kanemura

doi:10.1016/j.bbrc.2014.04.070

Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Yohei Bamba, Tomoko Shofuda, Daisuke Kanematsu, Masahiro Nonaka, Mami Yamasaki, Hideyuki Okano, Yonehiro Kanemura

Department of Physiology

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.

Original language	English
Pages (from-to)	683-688
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	447
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2014.04.070
Publication status	Published - 2014 May 16

Keywords

Glioma
Induced pluripotent stem cells
Neuronal migration
PiggyBac transposon
Radial glia
Regenerative medicine

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2014.04.070

Cite this

Bamba, Y., Shofuda, T., Kanematsu, D., Nonaka, M., Yamasaki, M., Okano, H., & Kanemura, Y. (2014). Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochemical and Biophysical Research Communications, 447(4), 683-688. https://doi.org/10.1016/j.bbrc.2014.04.070

Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. / Bamba, Yohei; Shofuda, Tomoko; Kanematsu, Daisuke et al.
In: Biochemical and Biophysical Research Communications, Vol. 447, No. 4, 16.05.2014, p. 683-688.

Research output: Contribution to journal › Article › peer-review

Bamba, Y, Shofuda, T, Kanematsu, D, Nonaka, M, Yamasaki, M, Okano, H & Kanemura, Y 2014, 'Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics', Biochemical and Biophysical Research Communications, vol. 447, no. 4, pp. 683-688. https://doi.org/10.1016/j.bbrc.2014.04.070

Bamba Y, Shofuda T, Kanematsu D, Nonaka M, Yamasaki M, Okano H et al. Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochemical and Biophysical Research Communications. 2014 May 16;447(4):683-688. doi: 10.1016/j.bbrc.2014.04.070

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abstract = "Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.",

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note = "Funding Information: We thank all the members of Dr. Kanemura{\textquoteright}s and Prof. Okano{\textquoteright}s laboratories. This study was supported by the Research Center Network for Realization of Regenerative Medicine, Japan Science and Technology Agency (JST), the JSPS KAKENHI Grant Number 24592181 , Japan, the Research on Applying Health Technology, Health and Labour Sciences Research Grants, the Ministry of Health, Labour and Welfare of Japan, and the Advanced Research for Medical Products Mining Programme of the National Institute of Biomedical Innovation (NIBIO). ",

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AU - Yamasaki, Mami

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AU - Kanemura, Yonehiro

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KW - Induced pluripotent stem cells

KW - Neuronal migration

KW - PiggyBac transposon

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Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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