Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

Shuji Kodama; Atsushi Yamamoto; Akinobu Matsunaga; Tomoyoshi Soga; Kazuichi Hayakawa

doi:10.1002/1522-2683(200109)22:15<3286::AID-ELPS3286>3.0.CO;2-J

Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

Shuji Kodama, Atsushi Yamamoto, Akinobu Matsunaga, Tomoyoshi Soga, Kazuichi Hayakawa

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mM copper(II) sulfate-1 mM L-tartrate (pH 5.1) with an effective voltage of-20 kV at 30°C, using direct detection at 280 nm, and resolution (R_s) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.

Original language	English
Pages (from-to)	3286-3290
Number of pages	5
Journal	ELECTROPHORESIS
Volume	22
Issue number	15
DOIs	https://doi.org/10.1002/1522-2683(200109)22:15<3286::AID-ELPS3286>3.0.CO;2-J
Publication status	Published - 2001
Externally published	Yes

Keywords

Apple juice
Capillary electrophoresis
Chiral resolution
Ligand exchange
Malic acid

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/1522-2683(200109)22:15<3286::AID-ELPS3286>3.0.CO;2-J

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title = "Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector",

abstract = "Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mM copper(II) sulfate-1 mM L-tartrate (pH 5.1) with an effective voltage of-20 kV at 30°C, using direct detection at 280 nm, and resolution (Rs) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.",

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author = "Shuji Kodama and Atsushi Yamamoto and Akinobu Matsunaga and Tomoyoshi Soga and Kazuichi Hayakawa",

year = "2001",

doi = "10.1002/1522-2683(200109)22:15<3286::AID-ELPS3286>3.0.CO;2-J",

language = "English",

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T1 - Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

AU - Kodama, Shuji

AU - Yamamoto, Atsushi

AU - Matsunaga, Akinobu

AU - Soga, Tomoyoshi

AU - Hayakawa, Kazuichi

PY - 2001

Y1 - 2001

N2 - Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mM copper(II) sulfate-1 mM L-tartrate (pH 5.1) with an effective voltage of-20 kV at 30°C, using direct detection at 280 nm, and resolution (Rs) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.

AB - Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mM copper(II) sulfate-1 mM L-tartrate (pH 5.1) with an effective voltage of-20 kV at 30°C, using direct detection at 280 nm, and resolution (Rs) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.

KW - Apple juice

KW - Capillary electrophoresis

KW - Chiral resolution

KW - Ligand exchange

KW - Malic acid

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Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

Abstract

Keywords

ASJC Scopus subject areas

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