Direct determination of p-hydroxymethamphetamine glucuronide in human urine by high-performance liquid chromatography

Rika Nakajima; Muneo Morishita; Sadakazu Aiso; Takehiko Yajima

doi:10.1248/cpb.54.493

Direct determination of p-hydroxymethamphetamine glucuronide in human urine by high-performance liquid chromatography

Rika Nakajima, Muneo Morishita, Sadakazu Aiso, Takehiko Yajima

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak® Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.

Original language	English
Pages (from-to)	493-495
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	54
Issue number	4
DOIs	https://doi.org/10.1248/cpb.54.493
Publication status	Published - 2006 Apr

Keywords

HPLC
Ion pair reagent
Solid-phase extraction
Urine sample
p-hydroxymethamphetamine glucuronide

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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abstract = "High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak{\textregistered} Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.",

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AU - Aiso, Sadakazu

AU - Yajima, Takehiko

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Direct determination of p-hydroxymethamphetamine glucuronide in human urine by high-performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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