Direct induction of neural cells from somatic cells

Wado Akamatsu; Hideyuki Okano

doi:10.1016/B978-0-12-813955-4.00013-1

Direct induction of neural cells from somatic cells

Wado Akamatsu, Hideyuki Okano

Department of Physiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Neural stem cells (NSCs) are a promising source for cell therapy in spinal cord injury (SCI). However, the preparation of NSCs from human-induced pluripotent stem cells (iPSCs) is time-consuming, and the preparation of autologous NSCs within the subacute phase after SCI is not possible at present. Previously, we succeeded in inducing self-renewing and tri-potent NSCs, which we designated induced NSCs (iNSCs), directly from mouse and human fibroblasts using Oct4, Sox2, Klf4, and c-Myc without clonal isolation of iPSCs. These iNSCs, which are generated without complete reprogramming into iPSCs, differentiate into neural lineage cells more rapidly than differentiation of similar cells from iPSCs. In addition to providing an overview of the generation and properties of iNSCs, we describe the application of new reprogramming technologies in neuroscience and disease modeling, in the derivation of induced neuronal cells.

Original language	English
Title of host publication	Rosenberg’s Molecular and Genetic Basis of Neurological and Psychiatric Disease
Subtitle of host publication	Volume 1
Publisher	Elsevier
Pages	179-185
Number of pages	7
ISBN (Electronic)	9780128139554
DOIs	https://doi.org/10.1016/B978-0-12-813955-4.00013-1
Publication status	Published - 2020 Jan 1

Keywords

Direct induction
Induced neuronal (iN) cells
Induced pluripotent stem cells (iPSCs)
Neural stem cells (NSCs)
Reprogramming

ASJC Scopus subject areas

Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/B978-0-12-813955-4.00013-1

Cite this

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abstract = "Neural stem cells (NSCs) are a promising source for cell therapy in spinal cord injury (SCI). However, the preparation of NSCs from human-induced pluripotent stem cells (iPSCs) is time-consuming, and the preparation of autologous NSCs within the subacute phase after SCI is not possible at present. Previously, we succeeded in inducing self-renewing and tri-potent NSCs, which we designated induced NSCs (iNSCs), directly from mouse and human fibroblasts using Oct4, Sox2, Klf4, and c-Myc without clonal isolation of iPSCs. These iNSCs, which are generated without complete reprogramming into iPSCs, differentiate into neural lineage cells more rapidly than differentiation of similar cells from iPSCs. In addition to providing an overview of the generation and properties of iNSCs, we describe the application of new reprogramming technologies in neuroscience and disease modeling, in the derivation of induced neuronal cells.",

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AU - Okano, Hideyuki

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KW - Direct induction

KW - Induced neuronal (iN) cells

KW - Induced pluripotent stem cells (iPSCs)

KW - Neural stem cells (NSCs)

KW - Reprogramming

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BT - Rosenberg’s Molecular and Genetic Basis of Neurological and Psychiatric Disease

PB - Elsevier

ER -

Direct induction of neural cells from somatic cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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