TY - GEN
T1 - Discrimination of normal skin fibroblast and malignant melanocytes using dielectrophoretic force and fluid-induced shear force
AU - Miyata, Shogo
AU - Ojima, Yuta
N1 - Publisher Copyright:
© 2014 TSI Press.
PY - 2014/10/24
Y1 - 2014/10/24
N2 - Cell analysis is an important technology that is widely used for medical diagnosis in hospitals and cell engineering research. Among cell analysis technology, dielectrophoresis (DEP) is one of the most promising approaches for discriminating biological particles because this phenomena requires no labeling procedure with a fluorescent dye or magnetic beads. In this study, we developed a precise cell analysis system by evaluating the DEP force with a liquid flow system. The DEP forces acting on a cell was characterized using a microfluidic chamber containing an electrode-array and fluid-induced shear forces. On the basis of this characterization, the discrimination between healthy skin cells and cancer cells was performed using our novel DEP cell sorting system. As a result, the healthy cells and cancer cells could be distinguished even if the dielectrophoretic properties of those cells were similar.
AB - Cell analysis is an important technology that is widely used for medical diagnosis in hospitals and cell engineering research. Among cell analysis technology, dielectrophoresis (DEP) is one of the most promising approaches for discriminating biological particles because this phenomena requires no labeling procedure with a fluorescent dye or magnetic beads. In this study, we developed a precise cell analysis system by evaluating the DEP force with a liquid flow system. The DEP forces acting on a cell was characterized using a microfluidic chamber containing an electrode-array and fluid-induced shear forces. On the basis of this characterization, the discrimination between healthy skin cells and cancer cells was performed using our novel DEP cell sorting system. As a result, the healthy cells and cancer cells could be distinguished even if the dielectrophoretic properties of those cells were similar.
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UR - http://www.scopus.com/inward/record.url?scp=84908893672&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908893672&partnerID=8YFLogxK
U2 - 10.1109/WAC.2014.6935706
DO - 10.1109/WAC.2014.6935706
M3 - Conference contribution
AN - SCOPUS:84908893672
T3 - World Automation Congress Proceedings
SP - 109
EP - 113
BT - World Automation Congress Proceedings
PB - IEEE Computer Society
T2 - 2014 World Automation Congress, WAC 2014
Y2 - 3 August 2014 through 7 August 2014
ER -