Disruption of hippocampal development in vivo by CR-50 mAB against reelin

Kazunori Nakajima, Katsuhiko Mikoshiba, Takaki Miyata, Chikako Kudo, Masaharu Ogawa

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156 Citations (Scopus)


We previously generated a monoclonal alloantibody, CR-50, by immunizing feeler mutant mice with homogenates of normal embryonic brains. This antibody recently was shown to recognize a Reelin protein, which is coded by the recently identified candidate gene for the reeler mutation. However, it is still unclear whether Reelin, especially the CR-50 epitope region, is indeed responsible for the reeler phenotype in vivo. Here we show that Reelin is localized on Cajal-Retzius neurons in the hippocampus and that intraventricular injection of CR-50 at the embryonic stage disrupts the organized development of the hippocampus in vivo, converting it to a reeler pattern. Labeling experiments with 5-bromodeoxyuridine demonstrated that the labeled cells in the stratum pyramidale of the CR-50-treated mice were distributed in a pattern similar to that of reeler. Thus, Cajal-Retzius neurons play a crucial function in hippocampus development, and the CR-50 epitope on Reelin plays a central role in this function.

Original languageEnglish
Pages (from-to)8196-8201
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number15
Publication statusPublished - 1997 Jul 22
Externally publishedYes

ASJC Scopus subject areas

  • General


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