Dissection of the auditory bulla in postnatal mice: Isolation of the middle ear bones and histological analysis

Ayako Sakamoto, Yukiko Kuroda, Sho Kanzaki, Koichi Matsuo

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called the auditory bulla houses the ossicles, whereas the auditory capsule encloses the inner ear, namely the cochlea and semicircular canals. Murine ossicles are essential for hearing and thus of great interest to researchers in the field of otolaryngology, but their metabolism, development, and evolution are highly relevant to other fields. Altered bone metabolism can affect hearing function in adult mice, and various gene-deficient mice show changes in morphogenesis of auditory ossicles in utero. Although murine auditory ossicles are tiny, their manipulation is feasible if one understands their anatomical orientation and 3D structure. Here, we describe how to dissect the auditory bulla and capsule of postnatal mice and then isolate individual ossicles by removing part of the bulla. We also discuss how to embed the bulla and capsule in different orientations to generate paraffin or frozen sections suitable for preparation of longitudinal, horizontal, or frontal sections of the malleus. Finally, we enumerate anatomical differences between mouse and human auditory ossicles. These methods would be useful in analyzing pathological, developmental and evolutionary aspects of auditory ossicles and the middle ear in mice.

Original languageEnglish
Article numbere55054
JournalJournal of Visualized Experiments
Issue number119
Publication statusPublished - 2017 Jan 4


  • Anatomy
  • Auditory ossicles
  • Cochlea
  • Incus
  • Inner ear
  • Issue 119
  • Malleus
  • Middle ear
  • Otic capsule
  • Stapedial artery
  • Stapes
  • Tensor tympani
  • Tympanic bulla
  • Tympanic membrane

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology


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