Distribution of the high-affinity choline transporter in the human and macaque monkey spinal cord

Yasushi Kobayashi, Takashi Okuda, Yasunori Fujioka, George Matsumura, Yu Nishimura, Tatsuya Haga

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


The distribution of the high-affinity choline transporter (CHT) was determined in the human and macaque monkey spinal cord using in situ hybridization histochemistry and immunohistochemistry. Signals for CHT mRNA were observed in somatic motor neurons, sympathetic preganglionic neurons, and neurons in the medial part of lamina VII. The mRNA for CHT was co-localized in single neurons with the mRNAs for vesicular acetylcholine transporter and cholineacetyltransferase. These same cholinergic neuronal groups were labeled by immunohistochemistry for human CHT. Of somatic motor neurons, smaller cell bodies of γ-motor neurons were labeled very intensely, whereas larger cell bodies of α-motor neurons showed various degrees of labeling from weak to moderately intense. Human CHT is thus a novel cholinergic marker, which not only labels cholinergic neurons, but also reveals their heterogeneity.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalNeuroscience Letters
Issue number1
Publication statusPublished - 2002 Jan 4
Externally publishedYes


  • Choline acetyltransferase
  • High-affinity choline trasnporter
  • Immunohistochemistry
  • In situ hybridization
  • Motor neurons
  • Vesicular acetylcholine trasnporter

ASJC Scopus subject areas

  • General Neuroscience


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