DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency

M. Hatakeyama; S. Iwato; K. Fujimoto; H. Handa; H. Kawaguchi

doi:10.1016/S0927-7765(97)00059-3

DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency

M. Hatakeyama, S. Iwato, K. Fujimoto, H. Handa, H. Kawaguchi

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Point mutation of specific genome DNA causes cancer. A system for rapid and sensitive detection of specific genome DNA is required because it enables early detection and treatment of cancer. We developed a new system for DNA diagnosis using DNA-carrying latex particles and succeeded to collect point mutant specifically from the mixture with normal DNA of codon 12 of K-ras gene.

Original language	English
Pages (from-to)	161-169
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/S0927-7765(97)00059-3
Publication status	Published - 1998 Feb 15

Keywords

Affinity latex
DNA diagnosis
Hybridization
Microsphere
Point mutation

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0927-7765(97)00059-3

Cite this

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title = "DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency",

abstract = "Point mutation of specific genome DNA causes cancer. A system for rapid and sensitive detection of specific genome DNA is required because it enables early detection and treatment of cancer. We developed a new system for DNA diagnosis using DNA-carrying latex particles and succeeded to collect point mutant specifically from the mixture with normal DNA of codon 12 of K-ras gene.",

keywords = "Affinity latex, DNA diagnosis, Hybridization, Microsphere, Point mutation",

author = "M. Hatakeyama and S. Iwato and K. Fujimoto and H. Handa and H. Kawaguchi",

note = "Funding Information: The authorsa cknowledgMe r M.Fukui and Mrs A.Kubota in Kyowa Medex Co. Ltd for helpful discussionT. he supportso f thisw ork by theG rant-in-Aid for ScientificR esearcho n Priority Areas {"}New Polymers and Their Nano-Organized Systems{"}( no. 277/08246246fr)o m the ministryo f Education, Science,S ports and Culture and the ResearchG rant of Keio Universitya re gratefully acknowledged.M H thanks the Japan Science Society for their support with Sasakawa ResearchG rant.",

year = "1998",

month = feb,

day = "15",

doi = "10.1016/S0927-7765(97)00059-3",

language = "English",

volume = "10",

pages = "161--169",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

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TY - JOUR

T1 - DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency

AU - Hatakeyama, M.

AU - Iwato, S.

AU - Fujimoto, K.

AU - Handa, H.

AU - Kawaguchi, H.

N1 - Funding Information: The authorsa cknowledgMe r M.Fukui and Mrs A.Kubota in Kyowa Medex Co. Ltd for helpful discussionT. he supportso f thisw ork by theG rant-in-Aid for ScientificR esearcho n Priority Areas "New Polymers and Their Nano-Organized Systems"( no. 277/08246246fr)o m the ministryo f Education, Science,S ports and Culture and the ResearchG rant of Keio Universitya re gratefully acknowledged.M H thanks the Japan Science Society for their support with Sasakawa ResearchG rant.

PY - 1998/2/15

Y1 - 1998/2/15

N2 - Point mutation of specific genome DNA causes cancer. A system for rapid and sensitive detection of specific genome DNA is required because it enables early detection and treatment of cancer. We developed a new system for DNA diagnosis using DNA-carrying latex particles and succeeded to collect point mutant specifically from the mixture with normal DNA of codon 12 of K-ras gene.

AB - Point mutation of specific genome DNA causes cancer. A system for rapid and sensitive detection of specific genome DNA is required because it enables early detection and treatment of cancer. We developed a new system for DNA diagnosis using DNA-carrying latex particles and succeeded to collect point mutant specifically from the mixture with normal DNA of codon 12 of K-ras gene.

KW - Affinity latex

KW - DNA diagnosis

KW - Hybridization

KW - Microsphere

KW - Point mutation

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U2 - 10.1016/S0927-7765(97)00059-3

DO - 10.1016/S0927-7765(97)00059-3

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AN - SCOPUS:0032519725

SN - 0927-7765

VL - 10

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 3

ER -

DNA-carrying latex particles for DNA diagnosis. 1. Separation of normal and point mutant DNAs according to the difference in hybridization efficiency

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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